The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00619087" target="_blank" >RIV/61389030:_____/25:00619087 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.crmeth.2025.101008" target="_blank" >https://doi.org/10.1016/j.crmeth.2025.101008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.crmeth.2025.101008" target="_blank" >10.1016/j.crmeth.2025.101008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research

Popis výsledku v původním jazyce

The development of canine immunotolerant monoclonal antibodies can accelerate the invention of new medicines for both canine and human diseases. We develop a methodology to clone the naive, somatically mutated variable domain repertoire from canine B cell mRNA using 50RACE PCR. A set of degenerate primers were then designed and used to clone variable domain genes into archival holding plasmid libraries. These archived variable domain genes were then combinatorially ligated to produce a scFv M13 phage library. Next- generation long-read and short-read DNA sequencing methodologies were developed to annotate features of the cloned library including CDR diversity and IGHV/IGKV/IGLV subfamily distribution. A synthetic immunoglobulin G was developed from this scFv library to the canine immune checkpoint receptor PD-1. This synthetic platform can be used to clone and annotate archived antibody variable domain genes for use in perpetuity in order to develop improved preclinical models for the treatment of complex human diseases.

Název v anglickém jazyce

The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research

Popis výsledku anglicky

The development of canine immunotolerant monoclonal antibodies can accelerate the invention of new medicines for both canine and human diseases. We develop a methodology to clone the naive, somatically mutated variable domain repertoire from canine B cell mRNA using 50RACE PCR. A set of degenerate primers were then designed and used to clone variable domain genes into archival holding plasmid libraries. These archived variable domain genes were then combinatorially ligated to produce a scFv M13 phage library. Next- generation long-read and short-read DNA sequencing methodologies were developed to annotate features of the cloned library including CDR diversity and IGHV/IGKV/IGLV subfamily distribution. A synthetic immunoglobulin G was developed from this scFv library to the canine immune checkpoint receptor PD-1. This synthetic platform can be used to clone and annotate archived antibody variable domain genes for use in perpetuity in order to develop improved preclinical models for the treatment of complex human diseases.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CELL REPORTS METHODS

ISSN

2667-2375

e-ISSN

2667-2375

Svazek periodika

5

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

101008

Kód UT WoS článku

001456387300001

EID výsledku v databázi Scopus

2-s2.0-105000456065