Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00627737" target="_blank" >RIV/61389030:_____/25:00627737 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00425-025-04699-w" target="_blank" >https://doi.org/10.1007/s00425-025-04699-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00425-025-04699-w" target="_blank" >10.1007/s00425-025-04699-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research

Popis výsledku v původním jazyce

In their paper published in 1967, Russell Jones and Joseph Varner (Planta 72: 155–161) developed a bioassay based on induction of α-amylase activity in barley embryo-less half-seeds that was specific for bioactive gibberellins. The induction of α-amylase in the aleurone of barley and other cereals was to become the experimental system of choice to study gibberellin signalling. However, despite much progress in identifying the molecular events linking gibberellin action and α-amylase gene expression, in many cases their role in the process is still unclear. In 1987, Steven Groot and Cees Karssen (Planta 171:525–531) showed that germination of tomato seeds was limited by the ability of the radicle to penetrate the surrounding layers, with the endosperm forming the major barrier. They used a modified needle attached to a tensiometer to measure the force required to break through the endosperm. While in wild-type seeds, a factor from the embryo, assumed to be gibberellin, promoted breakdown of the endosperm, gibberellin-deficient seeds required an external supply of the hormone to weaken the endosperm or for it to be mechanically disrupted for germination to occur. The paradigm of seed germination being physically restricted by surrounding layers and the role of gibberellin in weakening these tissues has been confirmed in many eudicot species. Gibberellin signalling induces the production of cell-wall loosening enzymes in the micropylar endosperm adjacent to the radicle, but it is unclear whether or not this is a direct response. In both eudicot and monocot systems, there is still much to learn about the role of gibberellin signalling in germination.

Název v anglickém jazyce

Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research

Popis výsledku anglicky

In their paper published in 1967, Russell Jones and Joseph Varner (Planta 72: 155–161) developed a bioassay based on induction of α-amylase activity in barley embryo-less half-seeds that was specific for bioactive gibberellins. The induction of α-amylase in the aleurone of barley and other cereals was to become the experimental system of choice to study gibberellin signalling. However, despite much progress in identifying the molecular events linking gibberellin action and α-amylase gene expression, in many cases their role in the process is still unclear. In 1987, Steven Groot and Cees Karssen (Planta 171:525–531) showed that germination of tomato seeds was limited by the ability of the radicle to penetrate the surrounding layers, with the endosperm forming the major barrier. They used a modified needle attached to a tensiometer to measure the force required to break through the endosperm. While in wild-type seeds, a factor from the embryo, assumed to be gibberellin, promoted breakdown of the endosperm, gibberellin-deficient seeds required an external supply of the hormone to weaken the endosperm or for it to be mechanically disrupted for germination to occur. The paradigm of seed germination being physically restricted by surrounding layers and the role of gibberellin in weakening these tissues has been confirmed in many eudicot species. Gibberellin signalling induces the production of cell-wall loosening enzymes in the micropylar endosperm adjacent to the radicle, but it is unclear whether or not this is a direct response. In both eudicot and monocot systems, there is still much to learn about the role of gibberellin signalling in germination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Planta

ISSN

0032-0935

e-ISSN

1432-2048

Svazek periodika

261

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

118

Kód UT WoS článku

001476088300001

EID výsledku v databázi Scopus

2-s2.0-105003821358