The transcription factor ENHANCER OF SHOOT REGENERATION 2 orchestrates cytokinin dynamics leading to developmental reprograming and green callus formation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637493" target="_blank" >RIV/61389030:_____/25:00637493 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1093/plphys/kiaf182" target="_blank" >https://doi.org/10.1093/plphys/kiaf182</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/plphys/kiaf182" target="_blank" >10.1093/plphys/kiaf182</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The transcription factor ENHANCER OF SHOOT REGENERATION 2 orchestrates cytokinin dynamics leading to developmental reprograming and green callus formation

Popis výsledku v původním jazyce

Callus formation and shoot regeneration are naturally triggered by stress and damage to the plant. They are also key components of tissue culture, which is crucial for gene editing, transformation, propagation, and other technologies in many species. Thus, the study of callus formation and shoot regeneration provides valuable insights into plant development. The transcription factor ENHANCER OF SHOOT REGENERATION 2/DORNROSCHEN-LIKE/BOLITA/SUPPRESSOR OF PHYTOCHROME B-4 2 (ESR2/DRNL/BOL/SOB2) promotes green callus formation in roots as well as shoot regeneration when overactive, and the phythormone cytokinin plays a prominent role in both the processes. Yet, the role of ESR2 in the cytokinin pathway has not been previously described in Arabidopsis (Arabidopsis thaliana). We found that cytokinin content and the expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE 5 (IPT5) are greater in plants with high ESR2 activity. ESR2 also regulates the cytokinin signaling repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), and surprisingly, ESR2-stimulated green callus formation requires IPT5 and AHP6. Therefore, ESR2 promotes both cytokinin biosynthesis and the inhibition of cytokinin signaling and, paradoxically, requires a combination of these 2 effects for green callus induction. These findings provide a foundation to better understand the processes involved in tissue reprograming toward callus formation and the role of ESR2 in callus formation and shoot regeneration.

Název v anglickém jazyce

The transcription factor ENHANCER OF SHOOT REGENERATION 2 orchestrates cytokinin dynamics leading to developmental reprograming and green callus formation

Popis výsledku anglicky

Callus formation and shoot regeneration are naturally triggered by stress and damage to the plant. They are also key components of tissue culture, which is crucial for gene editing, transformation, propagation, and other technologies in many species. Thus, the study of callus formation and shoot regeneration provides valuable insights into plant development. The transcription factor ENHANCER OF SHOOT REGENERATION 2/DORNROSCHEN-LIKE/BOLITA/SUPPRESSOR OF PHYTOCHROME B-4 2 (ESR2/DRNL/BOL/SOB2) promotes green callus formation in roots as well as shoot regeneration when overactive, and the phythormone cytokinin plays a prominent role in both the processes. Yet, the role of ESR2 in the cytokinin pathway has not been previously described in Arabidopsis (Arabidopsis thaliana). We found that cytokinin content and the expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE 5 (IPT5) are greater in plants with high ESR2 activity. ESR2 also regulates the cytokinin signaling repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), and surprisingly, ESR2-stimulated green callus formation requires IPT5 and AHP6. Therefore, ESR2 promotes both cytokinin biosynthesis and the inhibition of cytokinin signaling and, paradoxically, requires a combination of these 2 effects for green callus induction. These findings provide a foundation to better understand the processes involved in tissue reprograming toward callus formation and the role of ESR2 in callus formation and shoot regeneration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology

ISSN

0032-0889

e-ISSN

1532-2548

Svazek periodika

198

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

kiaf182

Kód UT WoS článku

001494023700001

EID výsledku v databázi Scopus

2-s2.0-105006945840