Cytokinin elevation caused by high light intensity contributes substantially to the increase of thermotolerance of rice plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637502" target="_blank" >RIV/61389030:_____/25:00637502 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/25:73634849

Výsledek na webu

<a href="https://doi.org/10.1016/j.stress.2025.100904" target="_blank" >https://doi.org/10.1016/j.stress.2025.100904</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.stress.2025.100904" target="_blank" >10.1016/j.stress.2025.100904</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin elevation caused by high light intensity contributes substantially to the increase of thermotolerance of rice plants

Popis výsledku v původním jazyce

Light is a crucial factor affecting plant development. Rice (Oryza sativa) responses to high light (HL, 1300 mu mol m-2 s-1, 27 degrees C) were characterized. HL significantly elevated root-born cytokinin, trans-zeatin in leaves due to transpiration stream. Despite only minor changes in abscisic acid (ABA) and jasmonic acid (JA) levels, HL decreased expression of ABA biosynthetic gene NCED5, while promoted JA signalling pathway by down-regulated expression of repressor JAZ9. The maximum quantum yield of photosystem II (Fv/Fm) decreased, suggesting protection of photosystem II. HL stimulated sugar production, especially glucose and mannose. As modulation of cytokinin levels (applying exogenous cytokinin or inhibitors of their degradation) promoted rice thermotolerance, HL treatment causing cytokinin increase was tested on its potential positive effect on response to elevated temperature. The effects of heat stress (HS, 40 degrees C for 6 h) per se and in combination with HL were evaluated in leaves, crowns and roots. HS was applied to whole plants (HS-WP), or targeted to leaves (HS-L) or roots (HS-R). HS treatments down-regulated trans-zeatin levels. Simultaneous application of HL and HS to leaves (HL+HS-WP, HL+HS-L) reduced this impact. HL combined with HS affecting leaves also stimulated JA and auxin indole-3-acetic acid. Milder expression of HS-marker genes HSP90.2, HSP90.3, HSP26.2 and HSFA2d, and genes coding antioxidant enzymes (especially Fe-superoxide dismutase) indicated that HL increased rice thermotolerance. HL minimized strong negative effect of HS on sugars in roots. The data showed positive impacts of mid-term HL treatment on thermotolerance of rice, revealing a novel strategy for stimulation of plant defence.

Název v anglickém jazyce

Cytokinin elevation caused by high light intensity contributes substantially to the increase of thermotolerance of rice plants

Popis výsledku anglicky

Light is a crucial factor affecting plant development. Rice (Oryza sativa) responses to high light (HL, 1300 mu mol m-2 s-1, 27 degrees C) were characterized. HL significantly elevated root-born cytokinin, trans-zeatin in leaves due to transpiration stream. Despite only minor changes in abscisic acid (ABA) and jasmonic acid (JA) levels, HL decreased expression of ABA biosynthetic gene NCED5, while promoted JA signalling pathway by down-regulated expression of repressor JAZ9. The maximum quantum yield of photosystem II (Fv/Fm) decreased, suggesting protection of photosystem II. HL stimulated sugar production, especially glucose and mannose. As modulation of cytokinin levels (applying exogenous cytokinin or inhibitors of their degradation) promoted rice thermotolerance, HL treatment causing cytokinin increase was tested on its potential positive effect on response to elevated temperature. The effects of heat stress (HS, 40 degrees C for 6 h) per se and in combination with HL were evaluated in leaves, crowns and roots. HS was applied to whole plants (HS-WP), or targeted to leaves (HS-L) or roots (HS-R). HS treatments down-regulated trans-zeatin levels. Simultaneous application of HL and HS to leaves (HL+HS-WP, HL+HS-L) reduced this impact. HL combined with HS affecting leaves also stimulated JA and auxin indole-3-acetic acid. Milder expression of HS-marker genes HSP90.2, HSP90.3, HSP26.2 and HSFA2d, and genes coding antioxidant enzymes (especially Fe-superoxide dismutase) indicated that HL increased rice thermotolerance. HL minimized strong negative effect of HS on sugars in roots. The data showed positive impacts of mid-term HL treatment on thermotolerance of rice, revealing a novel strategy for stimulation of plant defence.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Stress

ISSN

2667-064X

e-ISSN

2667-064X

Svazek periodika

16

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

100904

Kód UT WoS článku

001502259400001

EID výsledku v databázi Scopus

2-s2.0-105006731547