Resveratrol attenuates hepatic oxidative stress and preserves gut mucosal integrity in high-fat diet-fed rats by modulating antioxidant and anti-inflammatory pathways

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638594" target="_blank" >RIV/61389030:_____/25:00638594 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s41598-025-08450-z" target="_blank" >https://doi.org/10.1038/s41598-025-08450-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-08450-z" target="_blank" >10.1038/s41598-025-08450-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Resveratrol attenuates hepatic oxidative stress and preserves gut mucosal integrity in high-fat diet-fed rats by modulating antioxidant and anti-inflammatory pathways

Popis výsledku v původním jazyce

This study evaluated the protective effects of resveratrol against high-fat diet (HFD)-induced hepatic dysfunction and intestinal mucus layer depletion with a focus on antioxidant and anti-inflammatory mechanisms. HFD-fed rats were treated with resveratrol for 8 weeks, and various metabolic, molecular, and histological parameters were assessed. Resveratrol therapy significantly reduced body weight gain and adiposity, lowered plasma and hepatic oxidative stress markers, and restored endogenous antioxidant enzyme activity. Liver function markers including ALT, AST, and ALP were normalized in treated animals. RT-PCR analysis showed enhanced expression of lipid-metabolizing and antioxidant genes including PPAR alpha, CPT-1, Nrf2, and HO-1. Histological analysis revealed that resveratrol attenuated hepatic steatosis, collagen deposition, and fibrosis. Importantly, it preserved the goblet cell population within both intestinal crypts and villi, thereby maintaining the integrity of the gut-mucus barrier. These findings demonstrate that resveratrol exerts a multi-organ protective effect by simultaneously preserving intestinal mucus, improving hepatic antioxidant defenses, and reducing fibrosis. This study highlights a novel gut-liver axis mechanism for resveratrol action that extends beyond its well-known anti-obesity and mitochondrial benefits.

Název v anglickém jazyce

Resveratrol attenuates hepatic oxidative stress and preserves gut mucosal integrity in high-fat diet-fed rats by modulating antioxidant and anti-inflammatory pathways

Popis výsledku anglicky

This study evaluated the protective effects of resveratrol against high-fat diet (HFD)-induced hepatic dysfunction and intestinal mucus layer depletion with a focus on antioxidant and anti-inflammatory mechanisms. HFD-fed rats were treated with resveratrol for 8 weeks, and various metabolic, molecular, and histological parameters were assessed. Resveratrol therapy significantly reduced body weight gain and adiposity, lowered plasma and hepatic oxidative stress markers, and restored endogenous antioxidant enzyme activity. Liver function markers including ALT, AST, and ALP were normalized in treated animals. RT-PCR analysis showed enhanced expression of lipid-metabolizing and antioxidant genes including PPAR alpha, CPT-1, Nrf2, and HO-1. Histological analysis revealed that resveratrol attenuated hepatic steatosis, collagen deposition, and fibrosis. Importantly, it preserved the goblet cell population within both intestinal crypts and villi, thereby maintaining the integrity of the gut-mucus barrier. These findings demonstrate that resveratrol exerts a multi-organ protective effect by simultaneously preserving intestinal mucus, improving hepatic antioxidant defenses, and reducing fibrosis. This study highlights a novel gut-liver axis mechanism for resveratrol action that extends beyond its well-known anti-obesity and mitochondrial benefits.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA23-05389S" target="_blank" >GA23-05389S: Nové CB2 a BChE modulátory proti Parkinsonově chorobě a souvisejícím patologiím</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

25162

Kód UT WoS článku

001527619700029

EID výsledku v databázi Scopus

2-s2.0-105010600694