Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638664" target="_blank" >RIV/61389030:_____/25:00638664 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.plaphy.2025.110283" target="_blank" >https://doi.org/10.1016/j.plaphy.2025.110283</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2025.110283" target="_blank" >10.1016/j.plaphy.2025.110283</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

Popis výsledku v původním jazyce

It is assumed that the production of somatic embryos (SEs), which closely resemble fully mature zygotic embryos (ZEs), increases the chance of producing somatic plants equivalent to seedlings. Desiccation is crucial to reduce the water content of SEs and to initiate biochemical changes that support germination and conversion into plants. We investigated the biological, histological, biochemical and molecular changes in the SEs of hybrid larch Larix eurolepis exposed to desiccation at high relative humidity (RH, 98 %), reduced RH (59 %), or a combination of both. Desiccation at 98 % RH (D98) resulted in starch reduction, sucrose synthesis and a decrease in abscisic acid content without affecting germination and plant frequencies. In addition, proteomic results showed that D98 directed SE metabolism towards the acquisition of desiccation tolerance and energy storage. These modifications were proved to be favorable for SE germination. Desiccation at 59 % RH (D59) reduced the water content to a level corresponding to that of dried zygotic embryos. It could negatively impact the germination rate. The response of D59 SEs was directed towards the activation of defense mechanisms, while modifications favoring desiccation tolerance were suppressed. The treatment, which combined the two desiccation modes successively, yielded the positive effects of D98 while reducing the water content of SEs, similar to D59. Overall, our results provide new insights into the processes associated with the desiccation of hybrid larch SEs. We have developed an effective desiccation method resulting in a reduced water content comparable to that of dry ZE, while ensuring a high germination rate and successful conversion into plants.

Název v anglickém jazyce

Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

Popis výsledku anglicky

It is assumed that the production of somatic embryos (SEs), which closely resemble fully mature zygotic embryos (ZEs), increases the chance of producing somatic plants equivalent to seedlings. Desiccation is crucial to reduce the water content of SEs and to initiate biochemical changes that support germination and conversion into plants. We investigated the biological, histological, biochemical and molecular changes in the SEs of hybrid larch Larix eurolepis exposed to desiccation at high relative humidity (RH, 98 %), reduced RH (59 %), or a combination of both. Desiccation at 98 % RH (D98) resulted in starch reduction, sucrose synthesis and a decrease in abscisic acid content without affecting germination and plant frequencies. In addition, proteomic results showed that D98 directed SE metabolism towards the acquisition of desiccation tolerance and energy storage. These modifications were proved to be favorable for SE germination. Desiccation at 59 % RH (D59) reduced the water content to a level corresponding to that of dried zygotic embryos. It could negatively impact the germination rate. The response of D59 SEs was directed towards the activation of defense mechanisms, while modifications favoring desiccation tolerance were suppressed. The treatment, which combined the two desiccation modes successively, yielded the positive effects of D98 while reducing the water content of SEs, similar to D59. Overall, our results provide new insights into the processes associated with the desiccation of hybrid larch SEs. We have developed an effective desiccation method resulting in a reduced water content comparable to that of dry ZE, while ensuring a high germination rate and successful conversion into plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

1873-2690

Svazek periodika

229

Číslo periodika v rámci svazku

Dec

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

110283

Kód UT WoS článku

001612417000004

EID výsledku v databázi Scopus

2-s2.0-105012127153