Gibberellin- deactivating GA2OX enzymes act as a hub for auxin-gibberellin cross talk in Arabidopsis thaliana root growth regulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638724" target="_blank" >RIV/61389030:_____/25:00638724 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10500938 RIV/61989592:15310/25:73634799

Výsledek na webu

<a href="https://doi.org/10.1073/pnas.2425574122" target="_blank" >https://doi.org/10.1073/pnas.2425574122</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.2425574122" target="_blank" >10.1073/pnas.2425574122</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gibberellin- deactivating GA2OX enzymes act as a hub for auxin-gibberellin cross talk in Arabidopsis thaliana root growth regulation

Popis výsledku v původním jazyce

Plant bodies are built from immobile cells, making the regulation of cell expansion essential for growth, development, and adaptation. In roots, cell elongation executes the movement of the root tips through the soil. This process is tightly controlled by numerous signaling pathways. Among these, gibberellin and auxin signaling stand out for their contrasting effects on root growth, interacting through complex cross talk at multiple regulatory levels. Here, we reveal the molecular basis of the auxin-gibberellin cross talk in the model plant Arabidopsis thaliana. We show that the auxin signaling pathway steers the expression of GIBBERELLIN2-OXIDASES (GA2OXs), key gibberellin-deactivating enzymes in the root elongation zone (EZ). GA2OXs are negative regulators of root cell elongation, GA2OX8 overexpression decreases gibberellin levels and inhibits root cell elongation, in contrast, the ga2ox heptuple mutant roots show elevated gibberellin levels in the EZ and grow longer roots. Intriguingly, shoot-derived auxin can regulate GA2OX6 and GA2OX8 expression in roots, linking systemic auxin signaling to local gibberellin level modulation. Together, our findings identify GA2OX6 and GA2OX8 enzymes as key mediators of auxin-gibberellin cross talk, providing insights into their roles in root elongation. These results expand our understanding of how auxin integrates with gibberellin signaling to coordinate root development and growth dynamics.

Název v anglickém jazyce

Gibberellin- deactivating GA2OX enzymes act as a hub for auxin-gibberellin cross talk in Arabidopsis thaliana root growth regulation

Popis výsledku anglicky

Plant bodies are built from immobile cells, making the regulation of cell expansion essential for growth, development, and adaptation. In roots, cell elongation executes the movement of the root tips through the soil. This process is tightly controlled by numerous signaling pathways. Among these, gibberellin and auxin signaling stand out for their contrasting effects on root growth, interacting through complex cross talk at multiple regulatory levels. Here, we reveal the molecular basis of the auxin-gibberellin cross talk in the model plant Arabidopsis thaliana. We show that the auxin signaling pathway steers the expression of GIBBERELLIN2-OXIDASES (GA2OXs), key gibberellin-deactivating enzymes in the root elongation zone (EZ). GA2OXs are negative regulators of root cell elongation, GA2OX8 overexpression decreases gibberellin levels and inhibits root cell elongation, in contrast, the ga2ox heptuple mutant roots show elevated gibberellin levels in the EZ and grow longer roots. Intriguingly, shoot-derived auxin can regulate GA2OX6 and GA2OX8 expression in roots, linking systemic auxin signaling to local gibberellin level modulation. Together, our findings identify GA2OX6 and GA2OX8 enzymes as key mediators of auxin-gibberellin cross talk, providing insights into their roles in root elongation. These results expand our understanding of how auxin integrates with gibberellin signaling to coordinate root development and growth dynamics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

e-ISSN

1091-6490

Svazek periodika

122

Číslo periodika v rámci svazku

30

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

e2425574122

Kód UT WoS článku

001543977200001

EID výsledku v databázi Scopus

2-s2.0-105012218504