Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00640005" target="_blank" >RIV/61389030:_____/25:00640005 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/25:73634916

Výsledek na webu

<a href="https://doi.org/10.1111/pce.70103" target="_blank" >https://doi.org/10.1111/pce.70103</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pce.70103" target="_blank" >10.1111/pce.70103</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures

Popis výsledku v původním jazyce

Temperature sensing to adjust developmental rates and phenological responses to different climatic environments is critical for plant survival. Population-based thermal-time threshold models predict linear relationships between temperature and, for example, seed germination rates (speed), but the mechanisms are not known. Here, we used an integrative approach into the molecular biomechanical mechanisms underpinning a thermal-time model by combining Lepidium sativum micropylar endosperm (CAP) and radicle transcriptome analysis at defined heat units (generated by different time-temperature combinations) with corresponding CAP biomechanics. The thermal-time model delivered linear relationships with germination rates, but the underpinning biomechanical mechanisms of CAP weakening differed fundamentally between the optimal (24 degrees C-27 degrees C), sub-optimal (colder: 11 degrees C, 18 degrees C) and supra-optimal (warmer: 32 degrees C) temperatures. Chilling (11 degrees C) differed from other temperatures in that its CAP weakening inhibition was combined with altered CAP stiffness/elasticity. Differentially expressed cell wall remodelling protein (CWRP) genes associated with CAP weakening and/or stiffness/elasticity were identified using defined heat unit comparisons. Xyloglucans, galactomannans, pectins and UDP-sugar metabolism were major targets. Temperature regulation of CAP CWRP expression by DELAY-OF-GERMINATION-1 (DOG1) controls CAP weakening and seed germination. We conclude that distinct and temperature-specific molecular and biomechanical mechanisms underpin the apparently linear thermal-time responses during CAP weakening and seed germination.

Název v anglickém jazyce

Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures

Popis výsledku anglicky

Temperature sensing to adjust developmental rates and phenological responses to different climatic environments is critical for plant survival. Population-based thermal-time threshold models predict linear relationships between temperature and, for example, seed germination rates (speed), but the mechanisms are not known. Here, we used an integrative approach into the molecular biomechanical mechanisms underpinning a thermal-time model by combining Lepidium sativum micropylar endosperm (CAP) and radicle transcriptome analysis at defined heat units (generated by different time-temperature combinations) with corresponding CAP biomechanics. The thermal-time model delivered linear relationships with germination rates, but the underpinning biomechanical mechanisms of CAP weakening differed fundamentally between the optimal (24 degrees C-27 degrees C), sub-optimal (colder: 11 degrees C, 18 degrees C) and supra-optimal (warmer: 32 degrees C) temperatures. Chilling (11 degrees C) differed from other temperatures in that its CAP weakening inhibition was combined with altered CAP stiffness/elasticity. Differentially expressed cell wall remodelling protein (CWRP) genes associated with CAP weakening and/or stiffness/elasticity were identified using defined heat unit comparisons. Xyloglucans, galactomannans, pectins and UDP-sugar metabolism were major targets. Temperature regulation of CAP CWRP expression by DELAY-OF-GERMINATION-1 (DOG1) controls CAP weakening and seed germination. We conclude that distinct and temperature-specific molecular and biomechanical mechanisms underpin the apparently linear thermal-time responses during CAP weakening and seed germination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell and Environment

ISSN

0140-7791

e-ISSN

1365-3040

Svazek periodika

48

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

21

Strana od-do

8047-8067

Kód UT WoS článku

001544209000001

EID výsledku v databázi Scopus

2-s2.0-105012410367