Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00640019" target="_blank" >RIV/61389030:_____/25:00640019 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/25:73631919 RIV/61989592:15640/25:73631919

Výsledek na webu

<a href="https://doi.org/10.1093/pcp/pcaf077" target="_blank" >https://doi.org/10.1093/pcp/pcaf077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/pcp/pcaf077" target="_blank" >10.1093/pcp/pcaf077</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach

Popis výsledku v původním jazyce

Cereal plants possess a fibrous root system in which crown roots form the major component. Crown roots develop post-embryonically from the lower, mostly underground nodes of the stem base. A strict spatiotemporal regulation of gene expression governs this process. Much of the knowledge about signaling pathways controlling crown root initiation (CRI) and development comes from rice. However, distinct regulatory mechanisms may have evolved in other cereals to adapt to different habitats.In this study, using a Crown Root Inducible System (CRIS), we aimed to investigate the early molecular regulation of barley CRI. We revealed dynamic transcriptomic changes within the first 24 hours following auxin stimulation. Among the differentially expressed genes, we identified orthologs of important CRI regulators from other cereals, demonstrating that CRIS is suitable for uncovering genes involved in CRI. Further, ATAC-seq revealed that CRI relies on changes in chromatin accessibility near root development-related genes and within distal intergenic regions.Finally, we focused on two transcription factors, HvNAC013 and CBF12C, which likely play roles in both CRI and abiotic stresses. By performing DAP-seq, we determined their genome-wide binding sites and identified their potential downstream targets. Data suggest that CBF12C is a putative target of HvNAC013, along with other auxin-responsive genes implicated in CRI. We propose that HvNAC013 and CBF12C function as part of a transcription factor network involved in CRI and potentially modulate root architecture in response to environmental conditions. This study enhances our understanding of the CRI mechanism in barley.

Název v anglickém jazyce

Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach

Popis výsledku anglicky

Cereal plants possess a fibrous root system in which crown roots form the major component. Crown roots develop post-embryonically from the lower, mostly underground nodes of the stem base. A strict spatiotemporal regulation of gene expression governs this process. Much of the knowledge about signaling pathways controlling crown root initiation (CRI) and development comes from rice. However, distinct regulatory mechanisms may have evolved in other cereals to adapt to different habitats.In this study, using a Crown Root Inducible System (CRIS), we aimed to investigate the early molecular regulation of barley CRI. We revealed dynamic transcriptomic changes within the first 24 hours following auxin stimulation. Among the differentially expressed genes, we identified orthologs of important CRI regulators from other cereals, demonstrating that CRIS is suitable for uncovering genes involved in CRI. Further, ATAC-seq revealed that CRI relies on changes in chromatin accessibility near root development-related genes and within distal intergenic regions.Finally, we focused on two transcription factors, HvNAC013 and CBF12C, which likely play roles in both CRI and abiotic stresses. By performing DAP-seq, we determined their genome-wide binding sites and identified their potential downstream targets. Data suggest that CBF12C is a putative target of HvNAC013, along with other auxin-responsive genes implicated in CRI. We propose that HvNAC013 and CBF12C function as part of a transcription factor network involved in CRI and potentially modulate root architecture in response to environmental conditions. This study enhances our understanding of the CRI mechanism in barley.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant and Cell Physiology

ISSN

0032-0781

e-ISSN

1471-9053

Svazek periodika

66

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

20

Strana od-do

1284-1303

Kód UT WoS článku

001555698800001

EID výsledku v databázi Scopus

2-s2.0-105017055503