Membrane transport of root-borne trans-zeatin riboside maintains the cytokinin homeostasis in shoots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00642696" target="_blank" >RIV/61389030:_____/25:00642696 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00142035 RIV/00216208:11310/25:10510107 RIV/61989592:15310/25:73632021

Výsledek na webu

<a href="https://doi.org/10.1093/jxb/eraf369" target="_blank" >https://doi.org/10.1093/jxb/eraf369</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/eraf369" target="_blank" >10.1093/jxb/eraf369</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Membrane transport of root-borne trans-zeatin riboside maintains the cytokinin homeostasis in shoots

Popis výsledku v původním jazyce

Ribosylated forms of the plant hormones cytokinins (CKs) are the dominant CK species translocated over long distances. The irreplaceable role of root-to-shoot translocated trans-zeatin riboside in the mediation of shoot development implies the existence of a yet-uncharacterized CK riboside-specific membrane transport system. In this work, we report significant differences in the kinetics of the membrane transport of CK nucleobases and ribosides and the overall affinity of membrane-bound carriers towards the two CK forms. We further characterize the membrane transport of CK nucleobases and ribosides mediated by Arabidopsis EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3 (AtENT3) in tobacco BY-2 cells. Combining experimental data with computational modelling, we show that residues Tyr61 and Asp129, which are conserved among plant ENTs but not among ENTs from other species, are necessary for CK binding and that their mutation abolishes the ability of AtENT3 to transport CKs. Finally, we show that changes in AtENT3 have different effects on the concentrations of trans-zeatin riboside throughout Arabidopsis plants and on the overall CK concentrations in roots, implying that AtENT3 participates in both the long- and the short-distance transport of CKs.

Název v anglickém jazyce

Membrane transport of root-borne trans-zeatin riboside maintains the cytokinin homeostasis in shoots

Popis výsledku anglicky

Ribosylated forms of the plant hormones cytokinins (CKs) are the dominant CK species translocated over long distances. The irreplaceable role of root-to-shoot translocated trans-zeatin riboside in the mediation of shoot development implies the existence of a yet-uncharacterized CK riboside-specific membrane transport system. In this work, we report significant differences in the kinetics of the membrane transport of CK nucleobases and ribosides and the overall affinity of membrane-bound carriers towards the two CK forms. We further characterize the membrane transport of CK nucleobases and ribosides mediated by Arabidopsis EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3 (AtENT3) in tobacco BY-2 cells. Combining experimental data with computational modelling, we show that residues Tyr61 and Asp129, which are conserved among plant ENTs but not among ENTs from other species, are necessary for CK binding and that their mutation abolishes the ability of AtENT3 to transport CKs. Finally, we show that changes in AtENT3 have different effects on the concentrations of trans-zeatin riboside throughout Arabidopsis plants and on the overall CK concentrations in roots, implying that AtENT3 participates in both the long- and the short-distance transport of CKs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Svazek periodika

76

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

6723-6740

Kód UT WoS článku

001586176700001

EID výsledku v databázi Scopus

2-s2.0-105024664575