Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00644589" target="_blank" >RIV/61389030:_____/25:00644589 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10509657

Výsledek na webu

<a href="https://doi.org/10.3389/fpls.2025.1694769" target="_blank" >https://doi.org/10.3389/fpls.2025.1694769</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2025.1694769" target="_blank" >10.3389/fpls.2025.1694769</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications

Popis výsledku v původním jazyce

Introduction:nBased on previous reports of defense-related defects in the Arabidopsis loss-of-function (LOF) mutants of the EXO70B1 and EXO70B2 exocyst subunits, we investigated the underlying causes of these phenotypes.nMethods:nWe analyzed stomatal aperture states in both young and adult plants. As the exocyst is involved in the secretion to the cell wall, we examined cell wall composition, and we correlated these findings via a comprehensive mRNA expression analysis.nResults:nOur results revealed and discovered a more closed initial state of stomatal opening in exo70B mutants, as well as altered methylation and acetylation modifications of pectin and hemicellulose in the studied mutant lines. These changes in cell wall modifications may contribute to both compromised stomatal aperture-dependent and stomatal aperture-independent defense responses, as well as to the transcriptional activation of defense pathways observed in non-infected mutant plants at adult developmental stages. Several candidate genes involved in these processes were pinpointed using RNA-seq analysis.nDiscussion:nInterestingly, although the primary phenotypic and RNA-seq deviations in young mutant lines may be specific for each of the two EXO70B mutant lines, they exhibit converging gene expression profiles in later developmental stages. This convergence may reflect the shared evolutionary origin of the two Brassicaceae EXO70 isoforms by duplication from a common ancestral gene.

Název v anglickém jazyce

Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications

Popis výsledku anglicky

Introduction:nBased on previous reports of defense-related defects in the Arabidopsis loss-of-function (LOF) mutants of the EXO70B1 and EXO70B2 exocyst subunits, we investigated the underlying causes of these phenotypes.nMethods:nWe analyzed stomatal aperture states in both young and adult plants. As the exocyst is involved in the secretion to the cell wall, we examined cell wall composition, and we correlated these findings via a comprehensive mRNA expression analysis.nResults:nOur results revealed and discovered a more closed initial state of stomatal opening in exo70B mutants, as well as altered methylation and acetylation modifications of pectin and hemicellulose in the studied mutant lines. These changes in cell wall modifications may contribute to both compromised stomatal aperture-dependent and stomatal aperture-independent defense responses, as well as to the transcriptional activation of defense pathways observed in non-infected mutant plants at adult developmental stages. Several candidate genes involved in these processes were pinpointed using RNA-seq analysis.nDiscussion:nInterestingly, although the primary phenotypic and RNA-seq deviations in young mutant lines may be specific for each of the two EXO70B mutant lines, they exhibit converging gene expression profiles in later developmental stages. This convergence may reflect the shared evolutionary origin of the two Brassicaceae EXO70 isoforms by duplication from a common ancestral gene.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

16

Číslo periodika v rámci svazku

DEC 17

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

1694769

Kód UT WoS článku

001651464700001

EID výsledku v databázi Scopus

2-s2.0-105026762856