Condensin II mediates resistance to genotoxic stress and prevents mitotic defects in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F26%3A00648024" target="_blank" >RIV/61389030:_____/26:00648024 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1093/plphys/kiag022" target="_blank" >https://doi.org/10.1093/plphys/kiag022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/plphys/kiag022" target="_blank" >10.1093/plphys/kiag022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Condensin II mediates resistance to genotoxic stress and prevents mitotic defects in Arabidopsis

Popis výsledku v původním jazyce

Genome functions are regulated by chromatin and 3D chromosome organization. Dynamic condensation and relaxation of chromosomes during the cell cycle are largely controlled by the Condensin complexes. We mapped mutants in the Condensin II subunits SMC2A, CAP-D3 and CAP-H2 as hypersensitive to DNA-protein crosslink (DPC) inducers zebularine and ICRF-187. This suggested that Condensin II is required for resistance to genotoxic stress in Arabidopsis (Arabidopsis thaliana) and prompted us to explore the underlying phenotypes. We show that the role of Condensin II in resistance to zebularine is independent of DNA damage response signaling by SOG1 and homology-directed repair. Furthermore, we found that Arabidopsis Condensin II mutants have incompletely condensed mitotic chromosomes and show anaphase bridges. The anaphase bridges were more frequent upon treatment with zebularine or ICRF-187 and the duration of mitosis was prolonged. Altogether, we demonstrate that proper large-scale chromatin organization by Condensin II is important for resistance to DNA damage in Arabidopsis.

Název v anglickém jazyce

Condensin II mediates resistance to genotoxic stress and prevents mitotic defects in Arabidopsis

Popis výsledku anglicky

Genome functions are regulated by chromatin and 3D chromosome organization. Dynamic condensation and relaxation of chromosomes during the cell cycle are largely controlled by the Condensin complexes. We mapped mutants in the Condensin II subunits SMC2A, CAP-D3 and CAP-H2 as hypersensitive to DNA-protein crosslink (DPC) inducers zebularine and ICRF-187. This suggested that Condensin II is required for resistance to genotoxic stress in Arabidopsis (Arabidopsis thaliana) and prompted us to explore the underlying phenotypes. We show that the role of Condensin II in resistance to zebularine is independent of DNA damage response signaling by SOG1 and homology-directed repair. Furthermore, we found that Arabidopsis Condensin II mutants have incompletely condensed mitotic chromosomes and show anaphase bridges. The anaphase bridges were more frequent upon treatment with zebularine or ICRF-187 and the duration of mitosis was prolonged. Altogether, we demonstrate that proper large-scale chromatin organization by Condensin II is important for resistance to DNA damage in Arabidopsis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology

ISSN

0032-0889

e-ISSN

1532-2548

Svazek periodika

200

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

kiag022

Kód UT WoS článku

001686601900001

EID výsledku v databázi Scopus

2-s2.0-105029945409