Liquid chromatography-tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F10%3AA110107E" target="_blank" >RIV/61988987:17110/10:A110107E - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00843989:_____/10:00101263

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Liquid chromatography-tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients

Popis výsledku v původním jazyce

A LC-MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase l metabolites AM1, AM9, and AM4N in whole blood and lymphocates isolated on the Histopaque gradient. 200 micro of whole blood was preciitatedwith 10 mol/L zinc sulfute in acetonitrile/methanol (40:60, v/v) and lymphocatesisolated from 1,5 mL blood were wstracted with acetonitrile/methanol (40:60, v/v). The analytes and internal standard cyclosporine D were separated on RP column BEH C18, 2,1x50 mm, 1,7 micro using gradient LC-MS/MS analysis in positive electrospray mode. Time of analysis was 5 min. Linearity in blood was 5-2000 micro/L for CsA, AM1, and AM9; 2-500 micro/L for AM4N; and 2-500 micro/L for all substances in lymphocytes. Coefficient of variations was 1,8-9,8% and recovery was 92,0-110,0 %.

Název v anglickém jazyce

Liquid chromatography-tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients

Popis výsledku anglicky

A LC-MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase l metabolites AM1, AM9, and AM4N in whole blood and lymphocates isolated on the Histopaque gradient. 200 micro of whole blood was preciitatedwith 10 mol/L zinc sulfute in acetonitrile/methanol (40:60, v/v) and lymphocatesisolated from 1,5 mL blood were wstracted with acetonitrile/methanol (40:60, v/v). The analytes and internal standard cyclosporine D were separated on RP column BEH C18, 2,1x50 mm, 1,7 micro using gradient LC-MS/MS analysis in positive electrospray mode. Time of analysis was 5 min. Linearity in blood was 5-2000 micro/L for CsA, AM1, and AM9; 2-500 micro/L for AM4N; and 2-500 micro/L for all substances in lymphocytes. Coefficient of variations was 1,8-9,8% and recovery was 92,0-110,0 %.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/1A8655" target="_blank" >1A8655: Nové možnosti TDM cyklosporinu A a jeho metabolitů po transplantaci ledvin</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

J SEP SCI

ISSN

1615-9306

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

18.5. 2010

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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