Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F18%3AA1901Z8S" target="_blank" >RIV/61988987:17110/18:A1901Z8S - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/18:00101321 RIV/65269705:_____/18:00070322 RIV/00843989:_____/18:E0107125

Výsledek na webu

<a href="http://pps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=12&SID=D53mK5Vm5TXNmRbzT3u&page=1&doc=5" target="_blank" >http://pps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=12&SID=D53mK5Vm5TXNmRbzT3u&page=1&doc=5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijmm.2018.04.008" target="_blank" >10.1016/j.ijmm.2018.04.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Popis výsledku v původním jazyce

A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p &lt; 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p &lt; 0.01), including a higher prevalence of the colicin B determinant (p &lt; 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p &lt; 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p &lt; 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.

Název v anglickém jazyce

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Popis výsledku anglicky

A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p &lt; 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p &lt; 0.01), including a higher prevalence of the colicin B determinant (p &lt; 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p &lt; 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p &lt; 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30219 - Gastroenterology and hepatology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Medical Microbiology

ISSN

1438-4221

e-ISSN

1618-0607

Svazek periodika

308

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

498-504

Kód UT WoS článku

000440393900002

EID výsledku v databázi Scopus

2-s2.0-85046661589