Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F19%3AA2001YZL" target="_blank" >RIV/61988987:17110/19:A2001YZL - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00843989:_____/19:E0107723

Výsledek na webu

<a href="https://biomed.papers.upol.cz/artkey/bio-201901-0005_comparison_of_a_new_immunoassay_and_pcr-based_method_for_quantification_of_micrornas_in_whole_blood_a_pilot_me.php" target="_blank" >https://biomed.papers.upol.cz/artkey/bio-201901-0005_comparison_of_a_new_immunoassay_and_pcr-based_method_for_quantification_of_micrornas_in_whole_blood_a_pilot_me.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2018.080" target="_blank" >10.5507/bp.2018.080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

Popis výsledku v původním jazyce

Background. MicroRNAs (miRNAs) are new generation biomarkers used in oncology, cardiology, metabolic syndrome, obesity or in neurology. miRNAs are short non-coding RNA molecules that regulate gene expression in eukaryotes. Aim. To compare a new commercial method for establishing miRNA (imunoassay) with a commercial kit RT qPCR. Methods. RNA was isolated from whole blood samples obtained from four healthy volunteers. The isolates were liquated and miRNA-93-Sp and miRNA-23a-3p were measured independently with commercial hsa-miR-93-Sp miREIA and hsa-miR-23a-3p miREIA, and commercial RT-qPCR kits. Results. Both miRNAs had good analytical characteristics, very good correlation with RT qPCR. The results between immunoassay and RT qPCR did not statistically differ. A method based on ELISA was faster (2 h with ELISA vs. 3 h with qPCR) and had lower CV then a method based on RT qPCR (see more text). Conclusion. MicroRNAs from blood or derived fractions are particularly interesting candidates for routine laboratory applications.The immunoassay can be performed on any device that processes the ELISA plates and is therefore available in almost every laboratory.

Název v anglickém jazyce

Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

Popis výsledku anglicky

Background. MicroRNAs (miRNAs) are new generation biomarkers used in oncology, cardiology, metabolic syndrome, obesity or in neurology. miRNAs are short non-coding RNA molecules that regulate gene expression in eukaryotes. Aim. To compare a new commercial method for establishing miRNA (imunoassay) with a commercial kit RT qPCR. Methods. RNA was isolated from whole blood samples obtained from four healthy volunteers. The isolates were liquated and miRNA-93-Sp and miRNA-23a-3p were measured independently with commercial hsa-miR-93-Sp miREIA and hsa-miR-23a-3p miREIA, and commercial RT-qPCR kits. Results. Both miRNAs had good analytical characteristics, very good correlation with RT qPCR. The results between immunoassay and RT qPCR did not statistically differ. A method based on ELISA was faster (2 h with ELISA vs. 3 h with qPCR) and had lower CV then a method based on RT qPCR (see more text). Conclusion. MicroRNAs from blood or derived fractions are particularly interesting candidates for routine laboratory applications.The immunoassay can be performed on any device that processes the ELISA plates and is therefore available in almost every laboratory.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30500 - Other medical sciences

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1213-8118

e-ISSN

1804-7521

Svazek periodika

1

Číslo periodika v rámci svazku

163

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

39-44

Kód UT WoS článku

000458362500005

EID výsledku v databázi Scopus

2-s2.0-85062361913