Oxidační poškození DNA indukované v plasmidu DNA reakcí s chemickými látkami

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F08%3AA0900RJ1" target="_blank" >RIV/61988987:17310/08:A0900RJ1 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Oxidative damage of DNA induced in plasmid by the reactions with chemical agents

Popis výsledku v původním jazyce

The aim of the work was to study interactions of chemical agents with nucleic acids. A wide variety of chemicals can cause damage of macromolecules, particularly oxidative stress leads to DNA damage. In this study, we tested chemicals that can cause oxidative DNA damage at sugar-phosphate backbone level. For the study of the effects of chemical agents on DNA plasmid DNA was used. The method using plasmid DNA was based on the different mobility of various topological forms of plasmid DNA. The changes inconformation were detected by means of agarose gel electrophoresis. Significant increases in formation of DNA strand breaks in plasmid DNA were detected after incubation of plasmid DNA with KMnO4, K2S2O8, peroxiacetic acid and NaClO. The results also show the effect of metal ions on DNA damage. In the present work, the effects of the agents with promising antioxidant activity (N-acetyl-cysteine, mannitol, dimethylsulfoxide, etc.) on plasmid DNA strand breakage has been studied.

Název v anglickém jazyce

Oxidative damage of DNA induced in plasmid by the reactions with chemical agents

Popis výsledku anglicky

The aim of the work was to study interactions of chemical agents with nucleic acids. A wide variety of chemicals can cause damage of macromolecules, particularly oxidative stress leads to DNA damage. In this study, we tested chemicals that can cause oxidative DNA damage at sugar-phosphate backbone level. For the study of the effects of chemical agents on DNA plasmid DNA was used. The method using plasmid DNA was based on the different mobility of various topological forms of plasmid DNA. The changes inconformation were detected by means of agarose gel electrophoresis. Significant increases in formation of DNA strand breaks in plasmid DNA were detected after incubation of plasmid DNA with KMnO4, K2S2O8, peroxiacetic acid and NaClO. The results also show the effect of metal ions on DNA damage. In the present work, the effects of the agents with promising antioxidant activity (N-acetyl-cysteine, mannitol, dimethylsulfoxide, etc.) on plasmid DNA strand breakage has been studied.

Druh

C - Kapitola v odborné knize

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Environmental changes and biological assessment IV

ISBN

978-80-7368-451-8

Počet stran výsledku

5

Strana od-do

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Počet stran knihy

391

Název nakladatele

University of Ostrava

Místo vydání

Ostrava

Kód UT WoS kapitoly

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