Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F17%3AA1801ODC" target="_blank" >RIV/61988987:17310/17:A1801ODC - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/17:00100392

Výsledek na webu

<a href="http://dx.doi.org/10.1073/pnas.1712311114" target="_blank" >http://dx.doi.org/10.1073/pnas.1712311114</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.1712311114" target="_blank" >10.1073/pnas.1712311114</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid

Popis výsledku v původním jazyce

Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features areconserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania -like flagellum attachment zone, and a Trypanosoma cruzi -like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.

Název v anglickém jazyce

Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid

Popis výsledku anglicky

Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features areconserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania -like flagellum attachment zone, and a Trypanosoma cruzi -like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ISSN

0027-8424

e-ISSN

—

Svazek periodika

114

Číslo periodika v rámci svazku

44

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

11757-11762

Kód UT WoS článku

000414127400070

EID výsledku v databázi Scopus

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