Effect of bacteria on the degradation ability of Pleurotus ostreatus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F17%3AA1801ORQ" target="_blank" >RIV/61988987:17310/17:A1801ORQ - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/17:00474661

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.scitotenv.2017.01.171" target="_blank" >http://dx.doi.org/10.1016/j.scitotenv.2017.01.171</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2017.01.171" target="_blank" >10.1016/j.scitotenv.2017.01.171</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of bacteria on the degradation ability of Pleurotus ostreatus

Popis výsledku v původním jazyce

White-rot fungi are efficient degraders of lignin whose extracellular enzymes have a potential to degradeorganopollutants. In natural conditions these fungi enter into interactions with other organisms, which may affecttheir biodegradation capacity. The aim was to investigate the ability of Pleurotus ostreatus to form stable biofilmsand to test the capacity of the fungus to degrade Remazol Brilliant Blue R in mixed cultures with bacteria. Bacterialcounts were determined to see the behavior of the bacterium in the mixed culture with the fungus. In axenic conditions,the homogenized fungal mycelium was able to form an active biofilm which quickly degraded the dye. Theaddition of Pseudomonas fluorescens or Bacillus licheniformis bacteria at 106 CFU.mL-1 did not affect the decolorizationrate by 7-d-old fungal biofilms where the decolorization rate reached 90%. In contrast, when fragments of thefungal mycelium were used for inoculation to pre-formed biofilm of P. fluorescens, the biofilm was allowed to developfor one week's time, no decolorization of RBBR was observed and low activities of MnP and laccase were detected.The use of agar disks covered with fungal mycelium for the inoculation to pre-formed biofilm of P. fluorescens resultedin a fully developed biofilm that decolorized RBBR with similar efficiency as the pure P. ostreatus. The differencebetween the agar-disk- and homogenized-mycelium inoculated fungal biofilms was corroborated by the measurementof total fungal biofilm biomass that was 6-fold lower in the latter biofilm. Capability of the fungus to overcomethe competition of the bacterial biofilm thus depended on the type of fungal growth centres, where intact hyphaewere superior to the fragments of mycelium. A similar effect was not observed with the biofilms of B. licheniformiswhere the bacterial growth was less massive. The ability of P. ostreatus biofilms to resist massive bacterial stress wasdemonstrated.

Název v anglickém jazyce

Effect of bacteria on the degradation ability of Pleurotus ostreatus

Popis výsledku anglicky

White-rot fungi are efficient degraders of lignin whose extracellular enzymes have a potential to degradeorganopollutants. In natural conditions these fungi enter into interactions with other organisms, which may affecttheir biodegradation capacity. The aim was to investigate the ability of Pleurotus ostreatus to form stable biofilmsand to test the capacity of the fungus to degrade Remazol Brilliant Blue R in mixed cultures with bacteria. Bacterialcounts were determined to see the behavior of the bacterium in the mixed culture with the fungus. In axenic conditions,the homogenized fungal mycelium was able to form an active biofilm which quickly degraded the dye. Theaddition of Pseudomonas fluorescens or Bacillus licheniformis bacteria at 106 CFU.mL-1 did not affect the decolorizationrate by 7-d-old fungal biofilms where the decolorization rate reached 90%. In contrast, when fragments of thefungal mycelium were used for inoculation to pre-formed biofilm of P. fluorescens, the biofilm was allowed to developfor one week's time, no decolorization of RBBR was observed and low activities of MnP and laccase were detected.The use of agar disks covered with fungal mycelium for the inoculation to pre-formed biofilm of P. fluorescens resultedin a fully developed biofilm that decolorized RBBR with similar efficiency as the pure P. ostreatus. The differencebetween the agar-disk- and homogenized-mycelium inoculated fungal biofilms was corroborated by the measurementof total fungal biofilm biomass that was 6-fold lower in the latter biofilm. Capability of the fungus to overcomethe competition of the bacterial biofilm thus depended on the type of fungal growth centres, where intact hyphaewere superior to the fragments of mycelium. A similar effect was not observed with the biofilms of B. licheniformiswhere the bacterial growth was less massive. The ability of P. ostreatus biofilms to resist massive bacterial stress wasdemonstrated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SCI TOTAL ENVIRON

ISSN

0048-9697

e-ISSN

—

Svazek periodika

584-585

Číslo periodika v rámci svazku

APRIL

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

1114-1120

Kód UT WoS článku

000399358500109

EID výsledku v databázi Scopus

—