Lipid-polymorphism of plant chloroplast thylakoid membranes. Enhanced non-bilayer lipid phases associated with increased membrane permeability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F19%3AA2001X3U" target="_blank" >RIV/61988987:17310/19:A2001X3U - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12929" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12929</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.12929" target="_blank" >10.1111/ppl.12929</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipid-polymorphism of plant chloroplast thylakoid membranes. Enhanced non-bilayer lipid phases associated with increased membrane permeability

Popis výsledku v původním jazyce

Earlier experiments, using 31P-NMR and time-resolved merocyanine fluorescence spectroscopy, have shown that isolated intact, fully functional plant thylakoid membranes, in addition to the bilayer phase, contain three non-bilayer (or non-lamellar) lipid phases. It has also been shown that the lipid polymorphism of thylakoid membranes can be characterized by remarkable plasticity, i.e. by significant variations in 31P-NMR signatures. However, changes in the lipid-phase behaviour of thylakoids could not be assigned to changes in the overall membrane organization and the photosynthetic activity, as tested by circular dichroism and 77 K fluorescence emission spectroscopy and the magnitude of the variable fluorescence of photosystem II, which all showed only marginal variations. In this work, we investigated in more detail the temporal stability of the different lipid phases by recording 31P-NMR spectra on isolated thylakoid membranes that were suspended in sorbitol- or NaCl-based media.

Název v anglickém jazyce

Lipid-polymorphism of plant chloroplast thylakoid membranes. Enhanced non-bilayer lipid phases associated with increased membrane permeability

Popis výsledku anglicky

Earlier experiments, using 31P-NMR and time-resolved merocyanine fluorescence spectroscopy, have shown that isolated intact, fully functional plant thylakoid membranes, in addition to the bilayer phase, contain three non-bilayer (or non-lamellar) lipid phases. It has also been shown that the lipid polymorphism of thylakoid membranes can be characterized by remarkable plasticity, i.e. by significant variations in 31P-NMR signatures. However, changes in the lipid-phase behaviour of thylakoids could not be assigned to changes in the overall membrane organization and the photosynthetic activity, as tested by circular dichroism and 77 K fluorescence emission spectroscopy and the magnitude of the variable fluorescence of photosystem II, which all showed only marginal variations. In this work, we investigated in more detail the temporal stability of the different lipid phases by recording 31P-NMR spectra on isolated thylakoid membranes that were suspended in sorbitol- or NaCl-based media.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

166

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

10

Strana od-do

278-287

Kód UT WoS článku

000466108300022

EID výsledku v databázi Scopus

2-s2.0-85062711237