Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA21026C7" target="_blank" >RIV/61988987:17310/20:A21026C7 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0301462220301836?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0301462220301836?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpc.2020.106475" target="_blank" >10.1016/j.bpc.2020.106475</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles

Popis výsledku v původním jazyce

This article aims to explore interaction of nanoparticles ZnO, TiO2 and CeO2 with bovine serum albumin (BSA). The samples of nanoparticles were laboratory prepared and compared with commercial nanoparticles. Nanoparticles were characterized by SEM, zeta potential and size measurements. Adsorption of particles took place at pH where the zeta potential of the protein and nanoparticles was opposite. Then the zeta potential and size distribution were measured and the amount of protein needed to reduce the zeta potential of nanoparticles to zero was determined. The changes of BSA structure were also observed by Circular Dichroism (CD). The change of BSA structure after the adsorption on nanoparticles was confirmed. The content of BSA α-helix structure varies during experiments from 13 to 77% in dependence of concentration of nanoparticles. The interaction of TiO2 and BSA was confirmed also by the Surface Plasmon Resonance (SPR) technique. On laboratory prepared TiO2 was bound amount of 73,4 pg·mm−2.

Název v anglickém jazyce

Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles

Popis výsledku anglicky

This article aims to explore interaction of nanoparticles ZnO, TiO2 and CeO2 with bovine serum albumin (BSA). The samples of nanoparticles were laboratory prepared and compared with commercial nanoparticles. Nanoparticles were characterized by SEM, zeta potential and size measurements. Adsorption of particles took place at pH where the zeta potential of the protein and nanoparticles was opposite. Then the zeta potential and size distribution were measured and the amount of protein needed to reduce the zeta potential of nanoparticles to zero was determined. The changes of BSA structure were also observed by Circular Dichroism (CD). The change of BSA structure after the adsorption on nanoparticles was confirmed. The content of BSA α-helix structure varies during experiments from 13 to 77% in dependence of concentration of nanoparticles. The interaction of TiO2 and BSA was confirmed also by the Surface Plasmon Resonance (SPR) technique. On laboratory prepared TiO2 was bound amount of 73,4 pg·mm−2.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOPHYS CHEM

ISSN

0301-4622

e-ISSN

1873-4200

Svazek periodika

267

Číslo periodika v rámci svazku

December 2020

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000595259100010

EID výsledku v databázi Scopus

2-s2.0-85091121342