Interaction among clays and bovine serum albumin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA210279R" target="_blank" >RIV/61988987:17310/20:A210279R - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA01430C#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA01430C#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d0ra01430c" target="_blank" >10.1039/d0ra01430c</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction among clays and bovine serum albumin

Popis výsledku v původním jazyce

Interaction among bovine serum albumin and various clays including pure clay minerals and bentonite were studied with the aim to describe interaction process. The adsorption of albumin on the clays is strongly affected by the behavior of clays in the aquatic environment (hydrolysis and release of cations). Sufficient amount of albumin was adsorbed on the acid activated montmorillonite K10 (0.067 mg.mg-1) and on the Illite-Smectite (0.086 mg.mg-1). These clay minerals do not affect strongly the sorption solution parameters such as pH value and content of cations. Practically no adsorption was observed on the bentonite and vermiculite. Bentonite and vermiculite are subject to stronger interactions with water which cause the increase of pH value of the sorption solution and release of cations to the solution and thus they cause conformational changes of albumin, which was confirmed by circular dichroism measurements. Obtained results were confirmed by infrared spectrometry and thermal analysis as well. Interaction of studied materials with bovine serum albumin causes the reduction of particle size in the case of all studied clays except of vermiculite. Albumin attacks probably the clay structure during the adsorption, which cause the decrease of particle size. Presented work contributes to the knowledge about interaction of bovine serum albumin with clays in the field of influence of physico-chemical behaviour of clays in the solution on the interaction with albumin.

Název v anglickém jazyce

Interaction among clays and bovine serum albumin

Popis výsledku anglicky

Interaction among bovine serum albumin and various clays including pure clay minerals and bentonite were studied with the aim to describe interaction process. The adsorption of albumin on the clays is strongly affected by the behavior of clays in the aquatic environment (hydrolysis and release of cations). Sufficient amount of albumin was adsorbed on the acid activated montmorillonite K10 (0.067 mg.mg-1) and on the Illite-Smectite (0.086 mg.mg-1). These clay minerals do not affect strongly the sorption solution parameters such as pH value and content of cations. Practically no adsorption was observed on the bentonite and vermiculite. Bentonite and vermiculite are subject to stronger interactions with water which cause the increase of pH value of the sorption solution and release of cations to the solution and thus they cause conformational changes of albumin, which was confirmed by circular dichroism measurements. Obtained results were confirmed by infrared spectrometry and thermal analysis as well. Interaction of studied materials with bovine serum albumin causes the reduction of particle size in the case of all studied clays except of vermiculite. Albumin attacks probably the clay structure during the adsorption, which cause the decrease of particle size. Presented work contributes to the knowledge about interaction of bovine serum albumin with clays in the field of influence of physico-chemical behaviour of clays in the solution on the interaction with albumin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

2046-2069

Svazek periodika

10

Číslo periodika v rámci svazku

72

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

43927-43939

Kód UT WoS článku

000599804300002

EID výsledku v databázi Scopus

2-s2.0-85098459992