Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AEA" target="_blank" >RIV/61988987:17310/21:A2202AEA - isvavai.cz</a>

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/parasitology/article/differences-in-mitochondrial-nadh-dehydrogenase-activities-in-trypanosomatids/EA6B4A40B83A41E60C5D99B7E45AC446" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/differences-in-mitochondrial-nadh-dehydrogenase-activities-in-trypanosomatids/EA6B4A40B83A41E60C5D99B7E45AC446</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182020002425" target="_blank" >10.1017/S0031182020002425</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids

Popis výsledku v původním jazyce

Complex I (NADH dehydrogenase) is the first enzyme in the respiratory chain. It catalyses the electron transfer from NADH to ubiquinone that is associated with proton pumping out of the matrix. In this study, we characterized NADH dehydrogenase activity in seven monoxenous trypanosomatid species: Blechomonas ayalai, Herpetomonas tarakana, Kentomonas sorsogonicus, Leptomonas seymouri, Novymonas esmeraldas, Sergeia podlipaevi and Wallacemonas raviniae. We also investigated the subunit composition of the complex I in dixenous Phytomonas serpens, in which its presence and activity have been previously documented. In addition to P. serpens, the complex I is functionally active in N. esmeraldas and S. podlipaevi. We also identified 24-32 subunits of the complex I in individual species by using mass spectrometry. Among them, for the first time, we recognized several proteins of the mitochondrial DNA origin.

Název v anglickém jazyce

Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids

Popis výsledku anglicky

Complex I (NADH dehydrogenase) is the first enzyme in the respiratory chain. It catalyses the electron transfer from NADH to ubiquinone that is associated with proton pumping out of the matrix. In this study, we characterized NADH dehydrogenase activity in seven monoxenous trypanosomatid species: Blechomonas ayalai, Herpetomonas tarakana, Kentomonas sorsogonicus, Leptomonas seymouri, Novymonas esmeraldas, Sergeia podlipaevi and Wallacemonas raviniae. We also investigated the subunit composition of the complex I in dixenous Phytomonas serpens, in which its presence and activity have been previously documented. In addition to P. serpens, the complex I is functionally active in N. esmeraldas and S. podlipaevi. We also identified 24-32 subunits of the complex I in individual species by using mass spectrometry. Among them, for the first time, we recognized several proteins of the mitochondrial DNA origin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PARASITOLOGY

ISSN

0031-1820

e-ISSN

1469-8161

Svazek periodika

148

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1161-1170

Kód UT WoS článku

000674635600008

EID výsledku v databázi Scopus

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