Expansion and transformation of the minor spliceosomal system in the slime mold Physarum polycephalum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AFB" target="_blank" >RIV/61988987:17310/21:A2202AFB - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0960982221005972?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0960982221005972?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2021.04.050" target="_blank" >10.1016/j.cub.2021.04.050</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expansion and transformation of the minor spliceosomal system in the slime mold Physarum polycephalum

Popis výsledku v původním jazyce

Spliceosomal introns interrupt nuclear genes and are removed from RNA transcripts ('spliced'') by machinery called spliceosomes. Although the vast majority of spliceosomal introns are removed by the so-called major (or 'U2'') spliceosome, diverse eukaryotes also contain a rare second form, the minor ('U12'') spliceosome, and associated ('U12-type'') introns.(1-3) In all characterized species, U12-type introns are distinguished by several features, including being rare in the genome (similar to 0.5% of all introns),(4-6) containing extended evolutionarily conserved splicing motifs,(4,5,7,8) being generally ancient,(9,10) and being inefficiently spliced.(11-13) Here, we report a remarkable exception in the slime mold Physarum polycephalum. The P. polycephalum genome contains >20,000 U12-type introns-25 times more than any other species-enriched in a diversity of non-canonical splice boundaries as well as transformed splicing signals that appear to have co-evolved with the spliceosome due to massive gain of efficiently spliced U12-type introns. These results reveal an unappreciated dynamism of minor spliceosomal introns and spliceosomal introns in general.

Název v anglickém jazyce

Expansion and transformation of the minor spliceosomal system in the slime mold Physarum polycephalum

Popis výsledku anglicky

Spliceosomal introns interrupt nuclear genes and are removed from RNA transcripts ('spliced'') by machinery called spliceosomes. Although the vast majority of spliceosomal introns are removed by the so-called major (or 'U2'') spliceosome, diverse eukaryotes also contain a rare second form, the minor ('U12'') spliceosome, and associated ('U12-type'') introns.(1-3) In all characterized species, U12-type introns are distinguished by several features, including being rare in the genome (similar to 0.5% of all introns),(4-6) containing extended evolutionarily conserved splicing motifs,(4,5,7,8) being generally ancient,(9,10) and being inefficiently spliced.(11-13) Here, we report a remarkable exception in the slime mold Physarum polycephalum. The P. polycephalum genome contains >20,000 U12-type introns-25 times more than any other species-enriched in a diversity of non-canonical splice boundaries as well as transformed splicing signals that appear to have co-evolved with the spliceosome due to massive gain of efficiently spliced U12-type introns. These results reveal an unappreciated dynamism of minor spliceosomal introns and spliceosomal introns in general.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CURR BIOL

ISSN

0960-9822

e-ISSN

1879-0445

Svazek periodika

31

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

3125-3131

Kód UT WoS článku

000678562700011

EID výsledku v databázi Scopus

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