The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AFL" target="_blank" >RIV/61988987:17310/21:A2202AFL - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00555440 RIV/68378050:_____/21:00555440 RIV/00216208:11310/21:10430985

Výsledek na webu

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000664191500005" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000664191500005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/molbev/msab020" target="_blank" >10.1093/molbev/msab020</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba

Popis výsledku v původním jazyce

The transition of free-living organisms to parasitic organisms is a mysterious process that occurs in all major eukaryotic lineages. Parasites display seemingly unique features associated with their pathogenicity; however, it is important to distinguish ancestral preconditions to parasitism from truly new parasite-specific functions. Here, we sequenced the genome and transcriptome of anaerobic free-living Mastigamoeba balamuthi and performed phylogenomic analysis of four related members of the Archamoebae, including Entamoeba histolytica, an important intestinal pathogen of humans. We aimed to trace gene histories throughout the adaptation of the aerobic ancestor of Archamoebae to anaerobiosis and throughout the transition from a free-living to a parasitic lifestyle. These events were associated with massive gene losses that, in parasitic lineages, resulted in a reduction in structural features, complete losses of some metabolic pathways, and a reduction in metabolic complexity. By reconstructing the features of the common ancestor of Archamoebae, we estimated preconditions for the evolution of parasitism in this lineage. The ancestor could apparently form chitinous cysts, possessed proteolytic enzyme machinery, compartmentalized the sulfate activation pathway in mitochondrion-related organelles, and possessed the components for anaerobic energy metabolism.

Název v anglickém jazyce

The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba

Popis výsledku anglicky

The transition of free-living organisms to parasitic organisms is a mysterious process that occurs in all major eukaryotic lineages. Parasites display seemingly unique features associated with their pathogenicity; however, it is important to distinguish ancestral preconditions to parasitism from truly new parasite-specific functions. Here, we sequenced the genome and transcriptome of anaerobic free-living Mastigamoeba balamuthi and performed phylogenomic analysis of four related members of the Archamoebae, including Entamoeba histolytica, an important intestinal pathogen of humans. We aimed to trace gene histories throughout the adaptation of the aerobic ancestor of Archamoebae to anaerobiosis and throughout the transition from a free-living to a parasitic lifestyle. These events were associated with massive gene losses that, in parasitic lineages, resulted in a reduction in structural features, complete losses of some metabolic pathways, and a reduction in metabolic complexity. By reconstructing the features of the common ancestor of Archamoebae, we estimated preconditions for the evolution of parasitism in this lineage. The ancestor could apparently form chitinous cysts, possessed proteolytic enzyme machinery, compartmentalized the sulfate activation pathway in mitochondrion-related organelles, and possessed the components for anaerobic energy metabolism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MOL BIOL EVOL

ISSN

0737-4038

e-ISSN

1537-1719

Svazek periodika

38

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

20

Strana od-do

2240-2259

Kód UT WoS článku

000664191500005

EID výsledku v databázi Scopus

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