Salt stress induces paramylon accumulation and fine-tuning the macro-organization of thylakoid membranes in Euglena gracilis cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AX0" target="_blank" >RIV/61988987:17310/21:A2202AX0 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fpls.2021.725699/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpls.2021.725699/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2021.725699" target="_blank" >10.3389/fpls.2021.725699</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Salt stress induces paramylon accumulation and fine-tuning the macro-organization of thylakoid membranes in Euglena gracilis cells

Popis výsledku v původním jazyce

The effect of salt stress condition on the growth, morphology, photosynthetic performance and paramylon content were examined in the mixotrophic, unicellular, flagellate Euglena gracilis. We found that salt stress negatively influenced the cell growth, accompanied by a decrease of the chlorophyll content. Circular dichroism spectroscopy revealed changes in the macro-organization of pigment-protein complexes due to salt treatment, while small-angle neutron scattering (SANS) investigations suggested reduction in the thylakoid stacking, an effect confirmed by transmission electron microscopy. At the same time, analysis of the thylakoid membrane complexes using native-PAGE revealed no significant change in the composition of supercomplexes of the photosynthetic apparatus. Salt stress did not substantially affect the photosynthetic activity, as reflected by the facts that chlorophyll fluorescence yield, electron transport rate and energy transfer between the photosystems did not change considerably in the salt-grown cells. We have observed notable increases in the carotenoid-to-chlorophyll ratio and the accumulation of paramylon in the salt-treated cells. We propose that accumulation of storage polysaccharides and changes in the pigment composition and thylakoid membrane organization help the adaptation of Euglena gracilis cells to salt stress and contribute to the maintenance of cellular processes under stress conditions.

Název v anglickém jazyce

Salt stress induces paramylon accumulation and fine-tuning the macro-organization of thylakoid membranes in Euglena gracilis cells

Popis výsledku anglicky

The effect of salt stress condition on the growth, morphology, photosynthetic performance and paramylon content were examined in the mixotrophic, unicellular, flagellate Euglena gracilis. We found that salt stress negatively influenced the cell growth, accompanied by a decrease of the chlorophyll content. Circular dichroism spectroscopy revealed changes in the macro-organization of pigment-protein complexes due to salt treatment, while small-angle neutron scattering (SANS) investigations suggested reduction in the thylakoid stacking, an effect confirmed by transmission electron microscopy. At the same time, analysis of the thylakoid membrane complexes using native-PAGE revealed no significant change in the composition of supercomplexes of the photosynthetic apparatus. Salt stress did not substantially affect the photosynthetic activity, as reflected by the facts that chlorophyll fluorescence yield, electron transport rate and energy transfer between the photosystems did not change considerably in the salt-grown cells. We have observed notable increases in the carotenoid-to-chlorophyll ratio and the accumulation of paramylon in the salt-treated cells. We propose that accumulation of storage polysaccharides and changes in the pigment composition and thylakoid membrane organization help the adaptation of Euglena gracilis cells to salt stress and contribute to the maintenance of cellular processes under stress conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA19-13637S" target="_blank" >GA19-13637S: Vliv nedvouvrstevných lipidů a nelamelární fáze lipidů na strukturu, dynamiku a funkci tylakoidních membrán rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

12

Číslo periodika v rámci svazku

NOV 16 2021

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1-15

Kód UT WoS článku

000725707400001

EID výsledku v databázi Scopus

2-s2.0-85120446495