High genetic diversity of immunity genes in an expanding population of a highly mobile carnivore, the grey wolf Canis lupus, in Central Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202EAS" target="_blank" >RIV/61988987:17310/21:A2202EAS - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/21:10439453

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/ddi.13360" target="_blank" >https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/ddi.13360</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ddi.13360" target="_blank" >10.1111/ddi.13360</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High genetic diversity of immunity genes in an expanding population of a highly mobile carnivore, the grey wolf Canis lupus, in Central Europe

Popis výsledku v původním jazyce

Aim: The aim of the study was to assess the effect of changes in population size and migration on variation in functional immunity genes in the previously bottlenecked population of the grey wolf, Canis lupus. Location: Eastern/Central Europe: Poland, Czechia, Slovakia. Methods: We genotyped 7 immunity genes: three MHC-DLA genes (dog leuko- cyte antigen) and four Toll-like receptor (TLR) genes among 130 wolves originating from three populations: two lowland (Baltic and Central European), and highland Carpathian. We contrasted the population structure in immunity genes with a neu- tral structure based on 13 microsatellites, and we analysed signatures of selection in the immunity loci. Results: We found high overall genetic variance in immunity genes and no evidence for decreased diversity in the recently established populations. The population struc- ture in immunity loci was weak, with pairwise F ST lower than for neutral markers. Although the results of neutrality tests were not significant, we identified codons under selection, both positive and negative. Main Conclusions: We demonstrated that despite recent population expansion which is expected to result in decreased genetic diversity, the diversity of immunity genes in the newly established wolf population is similar to those in the source population. This suggests that migrations do not cause allele loss in grey wolf. Signatures of selec- tion on codon level, but not in tests using allele frequencies, suggest the contrasting effects of demography and selection.

Název v anglickém jazyce

High genetic diversity of immunity genes in an expanding population of a highly mobile carnivore, the grey wolf Canis lupus, in Central Europe

Popis výsledku anglicky

Aim: The aim of the study was to assess the effect of changes in population size and migration on variation in functional immunity genes in the previously bottlenecked population of the grey wolf, Canis lupus. Location: Eastern/Central Europe: Poland, Czechia, Slovakia. Methods: We genotyped 7 immunity genes: three MHC-DLA genes (dog leuko- cyte antigen) and four Toll-like receptor (TLR) genes among 130 wolves originating from three populations: two lowland (Baltic and Central European), and highland Carpathian. We contrasted the population structure in immunity genes with a neu- tral structure based on 13 microsatellites, and we analysed signatures of selection in the immunity loci. Results: We found high overall genetic variance in immunity genes and no evidence for decreased diversity in the recently established populations. The population struc- ture in immunity loci was weak, with pairwise F ST lower than for neutral markers. Although the results of neutrality tests were not significant, we identified codons under selection, both positive and negative. Main Conclusions: We demonstrated that despite recent population expansion which is expected to result in decreased genetic diversity, the diversity of immunity genes in the newly established wolf population is similar to those in the source population. This suggests that migrations do not cause allele loss in grey wolf. Signatures of selec- tion on codon level, but not in tests using allele frequencies, suggest the contrasting effects of demography and selection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

DIVERS DISTRIB

ISSN

1366-9516

e-ISSN

1472-4642

Svazek periodika

27

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

1680-1695

Kód UT WoS článku

000662972700001

EID výsledku v databázi Scopus

2-s2.0-85107953898