New foundations for the physical mechanism of variable chlorophyll a fluorescence. Quantum efficiency versus the light-adapted state of photosystem II

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F23%3AA2402L4I" target="_blank" >RIV/61988987:17310/23:A2402L4I - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/jxb/article-abstract/74/18/5458/7220642?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/jxb/article-abstract/74/18/5458/7220642?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erad252" target="_blank" >10.1093/jxb/erad252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New foundations for the physical mechanism of variable chlorophyll a fluorescence. Quantum efficiency versus the light-adapted state of photosystem II

Popis výsledku v původním jazyce

Photosystem II (PSII) uses solar energy to oxidize water and delivers electrons to fix CO2. Although the atomic resolution structure and basic photophysical and photochemical functions of PSII are well understood, many important questions remain. The activity of PSII in vitro and in vivo is routinely monitored by recording chlorophyll-a fluorescence induction kinetics (ChlF). According to the ‘mainstream’ model, the rise from the minimum level (Fo) to the maximum (Fm) of ChlF of dark-adapted PSII reflects the closure of all functionally active reaction centers, and the Fv/Fm ratio is equated with the maximum photochemical quantum yield of PSII (Fv=Fm–Fo). However, this model has never been free of controversies. A range of recent experimental data confirmed that the first single-turnover saturating flash (STSF), generating closed state (PSIIC), produces F1<Fm; and uncovered rate-limiting steps – Δτ1/2 half-waiting times – in the multi-STSF induced F1-to-Fm increments, originating from the gradual formation of light-adapted charge-separated state (PSIIL) with significantly increased stability of charges compared to PSIIC elicited by a single STSF. All data show that the interpretation of ChlF must be laid on new grounds. Here we discuss the underlying physical mechanisms and the significance of structural/functional dynamics of PSII – as reflected by ChlF and variations of the novel parameter Δτ1/2.

Název v anglickém jazyce

New foundations for the physical mechanism of variable chlorophyll a fluorescence. Quantum efficiency versus the light-adapted state of photosystem II

Popis výsledku anglicky

Photosystem II (PSII) uses solar energy to oxidize water and delivers electrons to fix CO2. Although the atomic resolution structure and basic photophysical and photochemical functions of PSII are well understood, many important questions remain. The activity of PSII in vitro and in vivo is routinely monitored by recording chlorophyll-a fluorescence induction kinetics (ChlF). According to the ‘mainstream’ model, the rise from the minimum level (Fo) to the maximum (Fm) of ChlF of dark-adapted PSII reflects the closure of all functionally active reaction centers, and the Fv/Fm ratio is equated with the maximum photochemical quantum yield of PSII (Fv=Fm–Fo). However, this model has never been free of controversies. A range of recent experimental data confirmed that the first single-turnover saturating flash (STSF), generating closed state (PSIIC), produces F1<Fm; and uncovered rate-limiting steps – Δτ1/2 half-waiting times – in the multi-STSF induced F1-to-Fm increments, originating from the gradual formation of light-adapted charge-separated state (PSIIL) with significantly increased stability of charges compared to PSIIC elicited by a single STSF. All data show that the interpretation of ChlF must be laid on new grounds. Here we discuss the underlying physical mechanisms and the significance of structural/functional dynamics of PSII – as reflected by ChlF and variations of the novel parameter Δτ1/2.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA23-07744S" target="_blank" >GA23-07744S: Identifikace a charakterizace struktur souvisejících s odlišnými fázemi lipidů tylakoidních membrán rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Svazek periodika

—

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

5458-5471

Kód UT WoS článku

001066729300001

EID výsledku v databázi Scopus

2-s2.0-85173036044