Lipid polymorphism of plant thylakoid membranes. The dynamic exchange model – facts and hypotheses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA2603AWC" target="_blank" >RIV/61988987:17310/25:A2603AWC - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.70230" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.70230</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.70230" target="_blank" >10.1111/ppl.70230</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipid polymorphism of plant thylakoid membranes. The dynamic exchange model – facts and hypotheses

Popis výsledku v původním jazyce

The light reactions of oxygenic photosynthesis are performed by protein complexes embedded in the lipid bilayer of thylakoid membranes (TMs). Bilayers provide optimal conditions for the build‐up of the proton motive force (pmf) and ATP synthesis. However, functional plant TMs, besides the bilayer, contain an inverted hexagonal (H<jats:sub>II</jats:sub>) phase and isotropic phases, a lipid polymorphism due to their major, non‐bilayer lipid species, monogalactosyldiacylglycerol (MGDG). The lipid phase behavior of TMs is explained within the framework of the Dynamic Exchange Model (DEM), an extension of the fluid‐mosaic model. DEM portrays the bilayer phase as inclusions between photosynthetic supercomplexes – characterized by compromised membrane impermeability and restricted sizes inflicted by the segregation propensity of lipid molecules, safe‐guarding the high protein density of TMs. Isotropic phases mediate membrane fusions and are associated with the lumenal lipocalin‐like enzyme, violaxanthin de‐epoxidase. Stromal‐side proteins surrounded by lipids give rise to the H<jats:sub>II</jats:sub> phase. These features instigate experimentally testable hypotheses: (i) non‐bilayer phases mediate functional sub‐compartmentalization of plant chloroplasts – a quasi‐autonomous energization and ATP synthesis of each granum‐stroma TM assembly; and (ii) the generation and utilization of pmf depend on hydrated protein networks and proton‐conducting pathways along membrane surfaces – rather than on strict impermeability of the bilayer.

Název v anglickém jazyce

Lipid polymorphism of plant thylakoid membranes. The dynamic exchange model – facts and hypotheses

Popis výsledku anglicky

The light reactions of oxygenic photosynthesis are performed by protein complexes embedded in the lipid bilayer of thylakoid membranes (TMs). Bilayers provide optimal conditions for the build‐up of the proton motive force (pmf) and ATP synthesis. However, functional plant TMs, besides the bilayer, contain an inverted hexagonal (H<jats:sub>II</jats:sub>) phase and isotropic phases, a lipid polymorphism due to their major, non‐bilayer lipid species, monogalactosyldiacylglycerol (MGDG). The lipid phase behavior of TMs is explained within the framework of the Dynamic Exchange Model (DEM), an extension of the fluid‐mosaic model. DEM portrays the bilayer phase as inclusions between photosynthetic supercomplexes – characterized by compromised membrane impermeability and restricted sizes inflicted by the segregation propensity of lipid molecules, safe‐guarding the high protein density of TMs. Isotropic phases mediate membrane fusions and are associated with the lumenal lipocalin‐like enzyme, violaxanthin de‐epoxidase. Stromal‐side proteins surrounded by lipids give rise to the H<jats:sub>II</jats:sub> phase. These features instigate experimentally testable hypotheses: (i) non‐bilayer phases mediate functional sub‐compartmentalization of plant chloroplasts – a quasi‐autonomous energization and ATP synthesis of each granum‐stroma TM assembly; and (ii) the generation and utilization of pmf depend on hydrated protein networks and proton‐conducting pathways along membrane surfaces – rather than on strict impermeability of the bilayer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA23-07744S" target="_blank" >GA23-07744S: Identifikace a charakterizace struktur souvisejících s odlišnými fázemi lipidů tylakoidních membrán rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PHYSIOL PLANTARUM

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

—

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

001469757600001

EID výsledku v databázi Scopus

2-s2.0-105003121158