Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27230%2F21%3A10249898" target="_blank" >RIV/61989100:27230/21:10249898 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/21:00075884 RIV/00216224:14110/21:00119394 RIV/00216305:26210/21:PU142368

Výsledek na webu

<a href="https://www.hindawi.com/journals/bmri/2021/6879765/" target="_blank" >https://www.hindawi.com/journals/bmri/2021/6879765/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2021/6879765" target="_blank" >10.1155/2021/6879765</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Popis výsledku v původním jazyce

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90 degrees periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180 degrees range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5.10(5) pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

Název v anglickém jazyce

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Popis výsledku anglicky

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90 degrees periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180 degrees range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5.10(5) pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomed Research International

ISSN

2314-6133

e-ISSN

2314-6141

Svazek periodika

2021

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

nestrankovano

Kód UT WoS článku

000771938700001

EID výsledku v databázi Scopus

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