Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27240%2F22%3A10250934" target="_blank" >RIV/61989100:27240/22:10250934 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-14627-5_39</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-14627-5_39" target="_blank" >10.1007/978-3-031-14627-5_39</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay

Popis výsledku v původním jazyce

The wound healing assay is one of the most widely used methods for studying cell migration and motility in vitro. In addition, modern automated equipment designed for time-lapse microscopy of living cells allows image sequences to be taken at short time intervals, enabling even more precise recording of cell migration dynamics. This paper describes the application of the procedure to evaluate image data acquired during wound healing of human chondrocytes and synovial sarcoma cells that were affected by the addition of synovial fluid from patients suffering from osteoarthritis. The proposed procedure is suitable for the analysis of wound healing assays even when the wound boundary cannot be detected by traditional methods, e.g. due to low cell density, numerous impurities, or when the distribution of cells forming the monolayer is affected by various factors present in the culture medium.

Název v anglickém jazyce

Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay

Popis výsledku anglicky

The wound healing assay is one of the most widely used methods for studying cell migration and motility in vitro. In addition, modern automated equipment designed for time-lapse microscopy of living cells allows image sequences to be taken at short time intervals, enabling even more precise recording of cell migration dynamics. This paper describes the application of the procedure to evaluate image data acquired during wound healing of human chondrocytes and synovial sarcoma cells that were affected by the addition of synovial fluid from patients suffering from osteoarthritis. The proposed procedure is suitable for the analysis of wound healing assays even when the wound boundary cannot be detected by traditional methods, e.g. due to low cell density, numerous impurities, or when the distribution of cells forming the monolayer is affected by various factors present in the culture medium.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Projekt

<a href="/cs/project/NU20-06-00269" target="_blank" >NU20-06-00269: Využití buněčných profilů a proteomiky synoviální tekutiny, případně tkání pro podporu klinického rozhodování u osteoartrózy kolena</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

ADVANCES IN INTELLIGENT NETWORKING AND COLLABORATIVE SYSTEMS, INCOS-2022

ISBN

978-3-031-14627-5

ISSN

2367-3370

e-ISSN

2367-3389

Počet stran výsledku

13

Strana od-do

380-392

Název nakladatele

SPRINGER INTERNATIONAL PUBLISHING AG

Místo vydání

CHAM

Místo konání akce

Kwansei Gakuin Univ

Datum konání akce

7. 9. 2022

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000870692600039