Combining extraction and cultivation methods for soil seed bank analysis increases number of captured species and their similarity to above-ground vegetation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27240%2F25%3A10257069" target="_blank" >RIV/61989100:27240/25:10257069 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27350/25:10257069

Výsledek na webu

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1500941/full" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1500941/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2024.1500941" target="_blank" >10.3389/fpls.2024.1500941</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combining extraction and cultivation methods for soil seed bank analysis increases number of captured species and their similarity to above-ground vegetation

Popis výsledku v původním jazyce

Introduction: Analysis of aboveground vegetation and soil seed bank is an important source of data on the state and dynamics of vegetation. It is especially important in landscapes exposed to disturbances, which have lost their functions. For our research, a post-mining area in the region of the Upper Silesian Black Coal Basin was selected, whose relief and ecosystems are strongly disturbed by underground mining and are currently also affected by ongoing climate change. Method: Data collection for our research took place in the territory of two waterlogged subsidence basins in the Karvina region, Czech Republic. We evaluated 30 phytosociological releves using techniques of Zurich – Montpellier school and 540 soil cores using cultivation and extraction method. Results: In the above-ground vegetation, 115 plant species were identified. By cultivating soil samples, we determined 60 species from 1,487 seedlings, by extraction method 66 species from 5,999 seeds. A statistically significant effect of the presence of the tree layer on the number of species obtained by the extraction method was demonstrated. There is also a statistically significant difference between the selected analysis methods in terms of the length of the captured seeds and their seed mass. Discussion: The construction of a rarefaction curve demonstrated that the use of cultivation and extraction methods leads to a greater capture of soil seed bank species. The similarity between the species composition of aboveground vegetation and the soil seed bank correspond to similarities observed in other studies from degraded habitats. Very low similarity between the species of the soil seed bank from cultivation and extraction method is probably caused by the highly variable distribution of seeds in the soil in time and space. Copyright © 2025 Plohák, Švehláková, Stalmachová, Goňo and Dvorský.

Název v anglickém jazyce

Combining extraction and cultivation methods for soil seed bank analysis increases number of captured species and their similarity to above-ground vegetation

Popis výsledku anglicky

Introduction: Analysis of aboveground vegetation and soil seed bank is an important source of data on the state and dynamics of vegetation. It is especially important in landscapes exposed to disturbances, which have lost their functions. For our research, a post-mining area in the region of the Upper Silesian Black Coal Basin was selected, whose relief and ecosystems are strongly disturbed by underground mining and are currently also affected by ongoing climate change. Method: Data collection for our research took place in the territory of two waterlogged subsidence basins in the Karvina region, Czech Republic. We evaluated 30 phytosociological releves using techniques of Zurich – Montpellier school and 540 soil cores using cultivation and extraction method. Results: In the above-ground vegetation, 115 plant species were identified. By cultivating soil samples, we determined 60 species from 1,487 seedlings, by extraction method 66 species from 5,999 seeds. A statistically significant effect of the presence of the tree layer on the number of species obtained by the extraction method was demonstrated. There is also a statistically significant difference between the selected analysis methods in terms of the length of the captured seeds and their seed mass. Discussion: The construction of a rarefaction curve demonstrated that the use of cultivation and extraction methods leads to a greater capture of soil seed bank species. The similarity between the species composition of aboveground vegetation and the soil seed bank correspond to similarities observed in other studies from degraded habitats. Very low similarity between the species of the soil seed bank from cultivation and extraction method is probably caused by the highly variable distribution of seeds in the soil in time and space. Copyright © 2025 Plohák, Švehláková, Stalmachová, Goňo and Dvorský.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

15

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

nestránkováno

Kód UT WoS článku

001411061000001

EID výsledku v databázi Scopus

2-s2.0-85216537026