Low-cost optical fiber sensors for the portable detection of cortisol and E. coli

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27240%2F25%3A10257794" target="_blank" >RIV/61989100:27240/25:10257794 - isvavai.cz</a>

Výsledek na webu

<a href="https://opg.optica.org/oe/fulltext.cfm?uri=oe-33-9-19851&id=570772" target="_blank" >https://opg.optica.org/oe/fulltext.cfm?uri=oe-33-9-19851&id=570772</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1364/OE.558813" target="_blank" >10.1364/OE.558813</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low-cost optical fiber sensors for the portable detection of cortisol and E. coli

Popis výsledku v původním jazyce

Detection of biomarkers, as well as bacterial infection levels, plays an important role in clinical analysis. Development in research for fast, high-sensitivity, and selective sensors has a key role in decision-making during a health issue. Aligning to these features, low-cost biomarkers and bacteria population detection methods should be developed in order to make clinical trials more accessible to the whole of society. In this context, optical sensors based on surface plasmon resonance (SPR) phenomena have been intensively studied due to their easy functionalization and fast and amplified response. This work aimed at the optical detection of some analytes in aqueous solutions. SPR-based D-shape sensors employing gold-deposited polymer optical fibers (POF) were used in a portable platform to detect concentrations of cortisol, the stress hormone, and E. coli, a bacteria strain. The sensors showed good linearity in response to increasing cortisol concentrations, but were less consistent in detecting the E. coli bacteria. However, E. coli sensors possess a sensitivity of-0.38 +/- 0.06 nm/log(CFU/mL), bearing a resemblance to the sensitivity obtained for cortisol sensors with-0.41 +/- 0.04 nm/log(ng/mL). The biosensors had limits of detection (LODs) lower than those of existing literature, with E. coli detection of 3.72 x 10-7 CFU/mL, and cortisol detection of 1.73 x 10-4 pg/mL. Small topography profile differences of about 2.0-3.4 nm were found through atomic force microscopy (AFM), equivalent to the thickness of the used antibodies, suggesting they were deposited parallel to the gold surface. In the future, methods with the potential to assure antibody verticality should be explored.

Název v anglickém jazyce

Low-cost optical fiber sensors for the portable detection of cortisol and E. coli

Popis výsledku anglicky

Detection of biomarkers, as well as bacterial infection levels, plays an important role in clinical analysis. Development in research for fast, high-sensitivity, and selective sensors has a key role in decision-making during a health issue. Aligning to these features, low-cost biomarkers and bacteria population detection methods should be developed in order to make clinical trials more accessible to the whole of society. In this context, optical sensors based on surface plasmon resonance (SPR) phenomena have been intensively studied due to their easy functionalization and fast and amplified response. This work aimed at the optical detection of some analytes in aqueous solutions. SPR-based D-shape sensors employing gold-deposited polymer optical fibers (POF) were used in a portable platform to detect concentrations of cortisol, the stress hormone, and E. coli, a bacteria strain. The sensors showed good linearity in response to increasing cortisol concentrations, but were less consistent in detecting the E. coli bacteria. However, E. coli sensors possess a sensitivity of-0.38 +/- 0.06 nm/log(CFU/mL), bearing a resemblance to the sensitivity obtained for cortisol sensors with-0.41 +/- 0.04 nm/log(ng/mL). The biosensors had limits of detection (LODs) lower than those of existing literature, with E. coli detection of 3.72 x 10-7 CFU/mL, and cortisol detection of 1.73 x 10-4 pg/mL. Small topography profile differences of about 2.0-3.4 nm were found through atomic force microscopy (AFM), equivalent to the thickness of the used antibodies, suggesting they were deposited parallel to the gold surface. In the future, methods with the potential to assure antibody verticality should be explored.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Optics Express

ISSN

1094-4087

e-ISSN

1094-4087

Svazek periodika

33

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

19851-19864

Kód UT WoS článku

001487064300002

EID výsledku v databázi Scopus

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