Combined spri sensor for simultaneous detection of nitrate and ammonium in wastewater

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27350%2F21%3A10246738" target="_blank" >RIV/61989100:27350/21:10246738 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27360/21:10246738 RIV/61989100:27710/21:10246738

Výsledek na webu

<a href="https://www.mdpi.com/1424-8220/21/3/725" target="_blank" >https://www.mdpi.com/1424-8220/21/3/725</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s21030725" target="_blank" >10.3390/s21030725</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combined spri sensor for simultaneous detection of nitrate and ammonium in wastewater

Popis výsledku v původním jazyce

Water pollution is a serious problem in modern society. Agriculture, being responsible for the discharge of agrochemicals, organic matter, or drug residues, produces a huge amount of wastewater. Aquaponics has the potential to reduce both water consumption and the impact of water pollution on fish farming and plant production. In the aquatic environment, inorganic nitrogen is mostly present in the form of nitrate and ammonium ions. Nitrate, as a final product of ammonia mineralization, is the most common chemical contaminant in aquifers around the world. For con-tinuous monitoring of nitrogen compounds in wastewater, we propose a sensor for the simultaneous detection of nitrate and ammonium. A surface plasmon resonance imaging method with en-zyme-mediated detection was used. Active layers of nitrate reductase and glutamine synthetase were created on the gold surface of a biochip and tested for the sensing of nitrate and ammonium in water from an aquaponic system. The proposed sensor was applied in water samples with a concentration of NO3MINUS SIGN and NH4+ in a range between 24-780 mgBULLET OPERATORLMINUS SIGN 1 and 0.26-120 mgBULLET OPERATORLMINUS SIGN 1, respectively, with minimal pretreatment of a sample by its dilution with a buffer prior to contact on a biochip surface. (C) 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Název v anglickém jazyce

Combined spri sensor for simultaneous detection of nitrate and ammonium in wastewater

Popis výsledku anglicky

Water pollution is a serious problem in modern society. Agriculture, being responsible for the discharge of agrochemicals, organic matter, or drug residues, produces a huge amount of wastewater. Aquaponics has the potential to reduce both water consumption and the impact of water pollution on fish farming and plant production. In the aquatic environment, inorganic nitrogen is mostly present in the form of nitrate and ammonium ions. Nitrate, as a final product of ammonia mineralization, is the most common chemical contaminant in aquifers around the world. For con-tinuous monitoring of nitrogen compounds in wastewater, we propose a sensor for the simultaneous detection of nitrate and ammonium. A surface plasmon resonance imaging method with en-zyme-mediated detection was used. Active layers of nitrate reductase and glutamine synthetase were created on the gold surface of a biochip and tested for the sensing of nitrate and ammonium in water from an aquaponic system. The proposed sensor was applied in water samples with a concentration of NO3MINUS SIGN and NH4+ in a range between 24-780 mgBULLET OPERATORLMINUS SIGN 1 and 0.26-120 mgBULLET OPERATORLMINUS SIGN 1, respectively, with minimal pretreatment of a sample by its dilution with a buffer prior to contact on a biochip surface. (C) 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20400 - Chemical engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors. Vol. 20

ISSN

1424-8220

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

000615502600001

EID výsledku v databázi Scopus

2-s2.0-85099543840