To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27360%2F25%3A10259727" target="_blank" >RIV/61989100:27360/25:10259727 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/smsc.202500446?getft_integrator=clarivate&src=getftr&utm_source=clarivate" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/smsc.202500446?getft_integrator=clarivate&src=getftr&utm_source=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/smsc.202500446" target="_blank" >10.1002/smsc.202500446</a>

Jazyk výsledku

angličtina

Název v původním jazyce

To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions

Popis výsledku v původním jazyce

Colloidally stable cerium oxide nanoparticles (CeNPs), known for mimicking multiple antioxidant enzymes, are promising nanozymes for therapeutic applications targeting oxidative stress and inflammation. Although fluorescent dye labeling facilitates nanoparticle imaging and tracking, its influence on physicochemical properties and biological interactions remains insufficiently understood. In this study, poly(acrylic acid)-coated CeNPs and their DiI-encapsulated counterparts are synthesized to evaluate the effects of dye functionalization on catalytic performance, cellular uptake, and intracellular fate. While overall redox cycling is retained, DiI@PAA-CeNPs show an 30% decrease in superoxide dismutase-like activity, whereas catalase-, peroxidase-, and oxidase-like activities remain largely preserved. Both formulations exhibit no cytotoxicity toward human osteoblasts. However, DiI labeling modifies surface chemistry and delays cellular uptake, particularly in the presence of serum proteins, as revealed by fluorescence microscopy. Multiscale imaging combining fluorescence microscopy, transmission electron microscopy, and focused ion beam-assisted lamella preparation reveals differences in nanoparticle internalization but similar intracellular fate. Focusing on poly(acrylic acid)-coated cerium oxide nanoparticles, DiI encapsulation can markedly alter CeNPs properties. This finding highlights the need to critically assess labeling effects in bio-nano studies. Integrating label-free techniques is crucial for accurate characterization and the rational design of nanozymes for diagnostic and therapeutic applications.

Název v anglickém jazyce

To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions

Popis výsledku anglicky

Colloidally stable cerium oxide nanoparticles (CeNPs), known for mimicking multiple antioxidant enzymes, are promising nanozymes for therapeutic applications targeting oxidative stress and inflammation. Although fluorescent dye labeling facilitates nanoparticle imaging and tracking, its influence on physicochemical properties and biological interactions remains insufficiently understood. In this study, poly(acrylic acid)-coated CeNPs and their DiI-encapsulated counterparts are synthesized to evaluate the effects of dye functionalization on catalytic performance, cellular uptake, and intracellular fate. While overall redox cycling is retained, DiI@PAA-CeNPs show an 30% decrease in superoxide dismutase-like activity, whereas catalase-, peroxidase-, and oxidase-like activities remain largely preserved. Both formulations exhibit no cytotoxicity toward human osteoblasts. However, DiI labeling modifies surface chemistry and delays cellular uptake, particularly in the presence of serum proteins, as revealed by fluorescence microscopy. Multiscale imaging combining fluorescence microscopy, transmission electron microscopy, and focused ion beam-assisted lamella preparation reveals differences in nanoparticle internalization but similar intracellular fate. Focusing on poly(acrylic acid)-coated cerium oxide nanoparticles, DiI encapsulation can markedly alter CeNPs properties. This finding highlights the need to critically assess labeling effects in bio-nano studies. Integrating label-free techniques is crucial for accurate characterization and the rational design of nanozymes for diagnostic and therapeutic applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21000 - Nano-technology

Projekt

<a href="/cs/project/EH22_008%2F0004631" target="_blank" >EH22_008/0004631: Materiály a technologie pro udržitelný rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Small Science

ISSN

2688-4046

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

nestránkováno

Kód UT WoS článku

001641626800001

EID výsledku v databázi Scopus

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