SURFACE-ENGINEERED METALLIC AND POLYMERIC MATERIALS PROMOTE OSTEOBLAST-LIKE CELL ADHESION, GROWTH AND DIFFERENTIATION FOR ORTHOPEDIC AND SPINE SURGERY APPLICATIONS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27640%2F25%3A10259343" target="_blank" >RIV/61989100:27640/25:10259343 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.scientific-publications.net/en/article/1002869/" target="_blank" >https://www.scientific-publications.net/en/article/1002869/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.62991/MMT1996712179" target="_blank" >10.62991/MMT1996712179</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SURFACE-ENGINEERED METALLIC AND POLYMERIC MATERIALS PROMOTE OSTEOBLAST-LIKE CELL ADHESION, GROWTH AND DIFFERENTIATION FOR ORTHOPEDIC AND SPINE SURGERY APPLICATIONS

Popis výsledku v původním jazyce

Materials commonly used in orthopedic, dental and spine surgeries—including Ti-6Al-4V alloy, stainless steel AISI 316 LWM, polyether ether ketone (PEEK) Optima, and ultra-high molecular weight polyethylene (UHMWPE)—were subjected to surface modifications such as sandblasting, polishing, and coating with amorphous hydrogenated carbon (a-C:H), alone or in combination. Surface roughness, assessed by the Ra parameter, was primarily at the submicron scale for unmodified or sandblasted materials and at the nanoscale for polished samples. Wettability and the polar component of surface energy were generally higher on metallic samples and standard polystyrene culture dishes than on polymeric materials, with the exception of polished stainless steel. When seeded with human osteoblast-like MG-63 cells, the highest cell densities were observed on sandblasted Ti-6Al-4V coated with a-C:H (day 1), polished stainless steel (day 3), and polished PEEK (day 8). These cells also displayed elevated levels of adhesion and osteogenic differentiation markers, including β1-integrins (on sandblasted Ti-6Al-4V with a-C:H), αv-integrins, vinculin, and osteocalcin (on polished stainless steel),and talin and osteopontin (on polished PEEK). Furthermore, the cells on all tested materials exhibited comparable or lower levels of intercellular adhesion molecule-1 (ICAM-1), an immune activation marker, compared to those grown on standard polystyrene dishes. These findings suggest that sandblasted Ti-6Al-4V coated with a-C:H, polished stainless steel, and polished PEEK are promising materials for bone implant construction and osseointegration

Název v anglickém jazyce

SURFACE-ENGINEERED METALLIC AND POLYMERIC MATERIALS PROMOTE OSTEOBLAST-LIKE CELL ADHESION, GROWTH AND DIFFERENTIATION FOR ORTHOPEDIC AND SPINE SURGERY APPLICATIONS

Popis výsledku anglicky

Materials commonly used in orthopedic, dental and spine surgeries—including Ti-6Al-4V alloy, stainless steel AISI 316 LWM, polyether ether ketone (PEEK) Optima, and ultra-high molecular weight polyethylene (UHMWPE)—were subjected to surface modifications such as sandblasting, polishing, and coating with amorphous hydrogenated carbon (a-C:H), alone or in combination. Surface roughness, assessed by the Ra parameter, was primarily at the submicron scale for unmodified or sandblasted materials and at the nanoscale for polished samples. Wettability and the polar component of surface energy were generally higher on metallic samples and standard polystyrene culture dishes than on polymeric materials, with the exception of polished stainless steel. When seeded with human osteoblast-like MG-63 cells, the highest cell densities were observed on sandblasted Ti-6Al-4V coated with a-C:H (day 1), polished stainless steel (day 3), and polished PEEK (day 8). These cells also displayed elevated levels of adhesion and osteogenic differentiation markers, including β1-integrins (on sandblasted Ti-6Al-4V with a-C:H), αv-integrins, vinculin, and osteocalcin (on polished stainless steel),and talin and osteopontin (on polished PEEK). Furthermore, the cells on all tested materials exhibited comparable or lower levels of intercellular adhesion molecule-1 (ICAM-1), an immune activation marker, compared to those grown on standard polystyrene dishes. These findings suggest that sandblasted Ti-6Al-4V coated with a-C:H, polished stainless steel, and polished PEEK are promising materials for bone implant construction and osseointegration

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

20500 - Materials engineering

Projekt

<a href="/cs/project/EH22_008%2F0004631" target="_blank" >EH22_008/0004631: Materiály a technologie pro udržitelný rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials, Methods &amp; Technologies. Volume 16

ISSN

1314-7269

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

19

Strana od-do

16-34

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—