Carbon dioxide or photosynthetically active radiation? Evaluation of the significance of individual environmental factors that control leaf stomatal development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27710%2F25%3A10259021" target="_blank" >RIV/61989100:27710/25:10259021 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:001615329000001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:001615329000001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/aob/mcaf249" target="_blank" >10.1093/aob/mcaf249</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carbon dioxide or photosynthetically active radiation? Evaluation of the significance of individual environmental factors that control leaf stomatal development

Popis výsledku v původním jazyce

Background and Aims Stomata are pores in the plant epidermis that regulate gas exchange by controlling CO(2 )uptake and water loss through transpiration. Stomatal conductance, which determines the rate of this gas exchange, is influenced by both the dynamic aperture of individual stomata and stomatal density (StD). It is a matter of discussion whether a single dominant factor that controls StD exists or a combination of several independent factors results in the final StD. Methods We evaluated the role of photosynthetic photon flux density (PPFD) and internal CO2 concentration (C-i). Plants of Helianthus annuus were cultivated under a gradient of PPFD in the range of 50-510 mu mol m(-2) s(-1); both cotyledons and true leaves were analysed. The independent effects of PPFD and C(i )on stomatal traits were assessed using additive linear models controlling for their physiological covariation. Results The results demonstrate that stomatal development in sunflower leaves is predominantly regulated by PPFD, not C-i. While stomatal traits in cotyledons remained largely unaffected by either PPFD or C-i, true leaves showed a clear and consistent response to light intensity. Additive linear models confirmed that only PPFD had a significant and robust effect on StD, whereas C-i showed no consistent contribution. This supports the interpretation that C-i, although physiologically coupled with PPFD, does not directly control stomatal development. Furthermore, we observed anatomical asymmetry between leaf surfaces: while both adaxial and abaxial sides responded to PPFD, the abaxial surface showed higher sensitivity, with significant effects on both StD and stomatal length. Conclusions Our findings emphasize light as the key environmental signal guiding stomatal patterning. Additionally, the results revealed varying differences in stomatal development on adaxial and abaxial sides of the leaf in response to light.

Název v anglickém jazyce

Carbon dioxide or photosynthetically active radiation? Evaluation of the significance of individual environmental factors that control leaf stomatal development

Popis výsledku anglicky

Background and Aims Stomata are pores in the plant epidermis that regulate gas exchange by controlling CO(2 )uptake and water loss through transpiration. Stomatal conductance, which determines the rate of this gas exchange, is influenced by both the dynamic aperture of individual stomata and stomatal density (StD). It is a matter of discussion whether a single dominant factor that controls StD exists or a combination of several independent factors results in the final StD. Methods We evaluated the role of photosynthetic photon flux density (PPFD) and internal CO2 concentration (C-i). Plants of Helianthus annuus were cultivated under a gradient of PPFD in the range of 50-510 mu mol m(-2) s(-1); both cotyledons and true leaves were analysed. The independent effects of PPFD and C(i )on stomatal traits were assessed using additive linear models controlling for their physiological covariation. Results The results demonstrate that stomatal development in sunflower leaves is predominantly regulated by PPFD, not C-i. While stomatal traits in cotyledons remained largely unaffected by either PPFD or C-i, true leaves showed a clear and consistent response to light intensity. Additive linear models confirmed that only PPFD had a significant and robust effect on StD, whereas C-i showed no consistent contribution. This supports the interpretation that C-i, although physiologically coupled with PPFD, does not directly control stomatal development. Furthermore, we observed anatomical asymmetry between leaf surfaces: while both adaxial and abaxial sides responded to PPFD, the abaxial surface showed higher sensitivity, with significant effects on both StD and stomatal length. Conclusions Our findings emphasize light as the key environmental signal guiding stomatal patterning. Additionally, the results revealed varying differences in stomatal development on adaxial and abaxial sides of the leaf in response to light.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Botany

ISSN

0305-7364

e-ISSN

1095-8290

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

November 2025

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

"zatím nepřiděleno"

Kód UT WoS článku

001615329000001

EID výsledku v databázi Scopus

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