Exprese, stabilita proteinu a transkripční aktivity receptorů pro kyselinu retinovou v primárních potkaních hepatocytech jsou ovlivněny látkami narušujícími mikrotubuly a all-trans retinovou kyselinou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F07%3A00003561" target="_blank" >RIV/61989592:15110/07:00003561 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes

Popis výsledku v původním jazyce

Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 ?M) down-regulated RAR? and RAR? mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RAR? mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RAR? < RAR? < RAR?. Thelevels of RAR? protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RAR? degradation. In contrast, the effects o

Název v anglickém jazyce

Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes

Popis výsledku anglicky

Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 ?M) down-regulated RAR? and RAR? mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RAR? mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RAR? < RAR? < RAR?. Thelevels of RAR? protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RAR? degradation. In contrast, the effects o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GP303%2F04%2FP074" target="_blank" >GP303/04/P074: Vliv pro a protizánětlivých faktorů na expresi cytochromu P450 - modulace přírodními látkami</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Endocrinology

ISSN

0303-7207

e-ISSN

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Svazek periodika

267

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

89-96

Kód UT WoS článku

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EID výsledku v databázi Scopus

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