Nové hodnocení oxidačního stresu v lidském semeni

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00006149" target="_blank" >RIV/61989592:15110/08:00006149 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A new approach to assess oxidative stress in human semen

Popis výsledku v původním jazyce

Introduction: Seminal oxidative stress (OS) has been implicated in the pathogenesis of male infertility. New laboratory methods are emerging to evaluate OS in human sperm. Reactive oxygen species (ROS) in human sperm were first measured in washed spermatozoa suspended in PBS using a chemiluminescence method. First reports of of levels of ROS in neat semen appeared only recently. The aim of our study was to assess ROS levels in both neat and processed semen using the same procedure and to compare the results in individual subjects. Material and Methods: Ejaculates from 68 males from infertile couples were evaluated together with control ejaculates from 18 fertile volunteers. ROS production was measured first in 400 ?l of neat semen and then in the samevolume of washed sperm suspension containing a fixed density of spermatozoa. The light emission of added luminol was measured for 15 min and expressed as RLU/min. Data were log-transformed and analysed using Spearman´s nonparametric test.

Název v anglickém jazyce

A new approach to assess oxidative stress in human semen

Popis výsledku anglicky

Introduction: Seminal oxidative stress (OS) has been implicated in the pathogenesis of male infertility. New laboratory methods are emerging to evaluate OS in human sperm. Reactive oxygen species (ROS) in human sperm were first measured in washed spermatozoa suspended in PBS using a chemiluminescence method. First reports of of levels of ROS in neat semen appeared only recently. The aim of our study was to assess ROS levels in both neat and processed semen using the same procedure and to compare the results in individual subjects. Material and Methods: Ejaculates from 68 males from infertile couples were evaluated together with control ejaculates from 18 fertile volunteers. ROS production was measured first in 400 ?l of neat semen and then in the samevolume of washed sperm suspension containing a fixed density of spermatozoa. The light emission of added luminol was measured for 15 min and expressed as RLU/min. Data were log-transformed and analysed using Spearman´s nonparametric test.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FK - Gynekologie a porodnictví

OECD FORD obor

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Projekt

<a href="/cs/project/1A8621" target="_blank" >1A8621: Oxidační stres v patofyziologii mužské neplodnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Andrology

ISSN

1802-4793

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

S1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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