Comparison of chemiluminesce and flow-cytometry for reactive oxygen species estimation in sperm suspension

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009819" target="_blank" >RIV/61989592:15110/09:00009819 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of chemiluminesce and flow-cytometry for reactive oxygen species estimation in sperm suspension

Popis výsledku v původním jazyce

Introduction: Sperm oxidative stress has been shown to detrimentally affect its lipid and protein content and to induce sperm DNA damage compromising sperm fertilizing ability. Superoxide, hydrogen peroxide and peroxynitrite are the common form of reactive oxygen species (ROS). Chemiluminescence using luminol measures both intracellular and extracellular ROS levels making it difficult to determine the source of the free radicals. Recently, flow-cytometry has been utilized to measure selectively intracellular ROS production with the added advantage of requiring smaller number of sperm. This study compared chemiluminescence and flow-cytometry in measuring sperm ROS production in a group of men within a large fertility clinic. Materials & methods: The study was approved by the Ethical Board of the Palacky University in Olomouc (CR). In order to included a broad spectrum of men the study recruited 166 men presenting with their partners for fertility assessment and 31 volunteers and sperm d

Název v anglickém jazyce

Comparison of chemiluminesce and flow-cytometry for reactive oxygen species estimation in sperm suspension

Popis výsledku anglicky

Introduction: Sperm oxidative stress has been shown to detrimentally affect its lipid and protein content and to induce sperm DNA damage compromising sperm fertilizing ability. Superoxide, hydrogen peroxide and peroxynitrite are the common form of reactive oxygen species (ROS). Chemiluminescence using luminol measures both intracellular and extracellular ROS levels making it difficult to determine the source of the free radicals. Recently, flow-cytometry has been utilized to measure selectively intracellular ROS production with the added advantage of requiring smaller number of sperm. This study compared chemiluminescence and flow-cytometry in measuring sperm ROS production in a group of men within a large fertility clinic. Materials & methods: The study was approved by the Ethical Board of the Palacky University in Olomouc (CR). In order to included a broad spectrum of men the study recruited 166 men presenting with their partners for fertility assessment and 31 volunteers and sperm d

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FK - Gynekologie a porodnictví

OECD FORD obor

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Projekt

<a href="/cs/project/1A8621" target="_blank" >1A8621: Oxidační stres v patofyziologii mužské neplodnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Human Reproduction

ISSN

0268-1161

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

Suppl 1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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