Site-specific Phosphorylation Dynamics of the Nuclear Proteome during the DNA Damage Response
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213211" target="_blank" >RIV/61989592:15110/10:10213211 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1074/mcp.M900616-MCP200" target="_blank" >http://dx.doi.org/10.1074/mcp.M900616-MCP200</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/mcp.M900616-MCP200" target="_blank" >10.1074/mcp.M900616-MCP200</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Site-specific Phosphorylation Dynamics of the Nuclear Proteome during the DNA Damage Response
Popis výsledku v původním jazyce
To investigate the temporal regulation of the DNA damage response, we applied quantitative mass spectrometry-based proteomics to measure site-specific phosphorylation changes of nuclear proteins after ionizing radiation. We profiled 5204 phosphorylationsites at five time points following DNA damage of which 594 sites on 209 proteins were observed to be regulated more than 2-fold. Of the 594 sites, 372 are novel phosphorylation sites primarily of nuclear origin. The 594 sites could be classified to distinct temporal profiles. Sites regulated shortly after radiation were enriched in the ataxia telangiectasia mutated (ATM) kinase SQ consensus sequence motif and a novel SXXQ motif. Importantly, in addition to induced phosphorylation, we identified a considerable group of sites that undergo DNA damage-induced dephosphorylation.
Název v anglickém jazyce
Site-specific Phosphorylation Dynamics of the Nuclear Proteome during the DNA Damage Response
Popis výsledku anglicky
To investigate the temporal regulation of the DNA damage response, we applied quantitative mass spectrometry-based proteomics to measure site-specific phosphorylation changes of nuclear proteins after ionizing radiation. We profiled 5204 phosphorylationsites at five time points following DNA damage of which 594 sites on 209 proteins were observed to be regulated more than 2-fold. Of the 594 sites, 372 are novel phosphorylation sites primarily of nuclear origin. The 594 sites could be classified to distinct temporal profiles. Sites regulated shortly after radiation were enriched in the ataxia telangiectasia mutated (ATM) kinase SQ consensus sequence motif and a novel SXXQ motif. Importantly, in addition to induced phosphorylation, we identified a considerable group of sites that undergo DNA damage-induced dephosphorylation.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular and Cellular Proteomics
ISSN
1535-9476
e-ISSN
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Svazek periodika
9
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
1314-1323
Kód UT WoS článku
000279396900021
EID výsledku v databázi Scopus
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