Neutrophilic differentiation modulates the apoptotic response of HL-60 cells to sodium butyrate and sodium valproate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213258" target="_blank" >RIV/61989592:15110/10:10213258 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Neutrophilic differentiation modulates the apoptotic response of HL-60 cells to sodium butyrate and sodium valproate

Popis výsledku v původním jazyce

We examined whether HL-60 cells differentiating by all-trans retinoic acid (ATRA) acquired resistance to apoptotic activity of butyrate and valproate. In undifferentiated cells, cytotoxicity of butyrate and valproate was associated with activation of intrinsic apoptotic pathway since we observed dissipation of mitochondrial potential, activation of caspase-9 and -3, appearance of sub-G1 DNA and loss of plasma membrane asymmetry and/or integrity. Both compounds also induced accumulation of undifferentiated cells in G0/G1 phase of cell cycle. ATRA enhanced the apoptotic effect of both butyrate and valproate in undifferentiated cells. This aside, ATRA synergized with butyrate in the induction of G0/G1 arrest. In cells pretreated for 72 h with ATRA, butyrate and valproate in combination with ATRA induced lower dissipation of mitochondrial membrane potential and weaker apoptotic and/or necrotic changes in plasma membrane, whereas DNA fragmentation was not diminished compared to undifferenti

Název v anglickém jazyce

Neutrophilic differentiation modulates the apoptotic response of HL-60 cells to sodium butyrate and sodium valproate

Popis výsledku anglicky

We examined whether HL-60 cells differentiating by all-trans retinoic acid (ATRA) acquired resistance to apoptotic activity of butyrate and valproate. In undifferentiated cells, cytotoxicity of butyrate and valproate was associated with activation of intrinsic apoptotic pathway since we observed dissipation of mitochondrial potential, activation of caspase-9 and -3, appearance of sub-G1 DNA and loss of plasma membrane asymmetry and/or integrity. Both compounds also induced accumulation of undifferentiated cells in G0/G1 phase of cell cycle. ATRA enhanced the apoptotic effect of both butyrate and valproate in undifferentiated cells. This aside, ATRA synergized with butyrate in the induction of G0/G1 arrest. In cells pretreated for 72 h with ATRA, butyrate and valproate in combination with ATRA induced lower dissipation of mitochondrial membrane potential and weaker apoptotic and/or necrotic changes in plasma membrane, whereas DNA fragmentation was not diminished compared to undifferenti

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neoplasma

ISSN

0028-2685

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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