Protopine and allocryptopine increase mRNA levels of cytochromes P450 1A in human hepatocytes and HepG2 cells independently of AhR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10222122" target="_blank" >RIV/61989592:15110/11:10222122 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protopine and allocryptopine increase mRNA levels of cytochromes P450 1A in human hepatocytes and HepG2 cells independently of AhR

Popis výsledku v původním jazyce

We examined whether alkaloids protopine or allocryptopine affect the expression of cytochromes P450 (CYPs) 1A1 and 1A2 in human hepatocytes and HepG2 cells. In HepG2 cells, protopine and allocryptopine increased CYP1A1 mRNA levels at concentrations from25 and 10 ?M, respectively. Both protopine and allocryptopine also increased CYP1A1 and CYP1A2 mRNA levels in human hepatocytes. Using gene reporter assays performed in transiently transfected HepG2 cells, we demonstrated that the induction of CYP1A1 expression by either protopine or allocryptopine was associated with negligible activation of aryl hydrocarbon receptor. CYP1A mRNA levels induced by protopine or allocryptopine in both HepG2 cells and human hepatocytes did not result in elevated CYP1A protein or activity levels. We conclude that the use of products containing protopine or allocryptopine may be considered safe in terms of possible induction of CYP1A enzymes.

Název v anglickém jazyce

Protopine and allocryptopine increase mRNA levels of cytochromes P450 1A in human hepatocytes and HepG2 cells independently of AhR

Popis výsledku anglicky

We examined whether alkaloids protopine or allocryptopine affect the expression of cytochromes P450 (CYPs) 1A1 and 1A2 in human hepatocytes and HepG2 cells. In HepG2 cells, protopine and allocryptopine increased CYP1A1 mRNA levels at concentrations from25 and 10 ?M, respectively. Both protopine and allocryptopine also increased CYP1A1 and CYP1A2 mRNA levels in human hepatocytes. Using gene reporter assays performed in transiently transfected HepG2 cells, we demonstrated that the induction of CYP1A1 expression by either protopine or allocryptopine was associated with negligible activation of aryl hydrocarbon receptor. CYP1A mRNA levels induced by protopine or allocryptopine in both HepG2 cells and human hepatocytes did not result in elevated CYP1A protein or activity levels. We conclude that the use of products containing protopine or allocryptopine may be considered safe in terms of possible induction of CYP1A enzymes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Svazek periodika

203

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

7

Strana od-do

135-141

Kód UT WoS článku

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EID výsledku v databázi Scopus

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