Oxidation of Sanguinarine and Its Dihydro-Derivative at a Pyrolytic Graphite Electrode Using Ex Situ Voltammetry. Study of the Interactions of the Alkaloids with DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10222937" target="_blank" >RIV/61989592:15110/11:10222937 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/11:00370858 RIV/61989592:15310/11:10222937

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Oxidation of Sanguinarine and Its Dihydro-Derivative at a Pyrolytic Graphite Electrode Using Ex Situ Voltammetry. Study of the Interactions of the Alkaloids with DNA

Popis výsledku v původním jazyce

This study describes oxidation of sanguinarine (SG) and its metabolite dihydrosanguinarine (DHSG) on the surface of a basal-plane pyrolytic graphite electrode (PGE). Since both alkaloids strongly adsorb onto the surface of pyrolytic graphite, measurements were performed using ex situ voltammetric methods, adsorptive transfer (AdT) cyclic voltammetry (CV) and square-wave voltammetry (SWV). The electrochemical results and optimized AdT SWV were subsequently applied for study of the interactions of SG andDHSG with DNA in vitro. Electrochemical study of the interactions was supported and complemented with measurements using gel electrophoresis and fluorescence spectroscopy. The results suggest that SG intercalates into the double-stranded structure of scDNA while increased binding affinity was observed for quaternary cation (SG+). DHSG which, unlike SG+, does not possess a strictly planar molecular structure, did not show intercalative DNA binding in any of the three methods applied.

Název v anglickém jazyce

Oxidation of Sanguinarine and Its Dihydro-Derivative at a Pyrolytic Graphite Electrode Using Ex Situ Voltammetry. Study of the Interactions of the Alkaloids with DNA

Popis výsledku anglicky

This study describes oxidation of sanguinarine (SG) and its metabolite dihydrosanguinarine (DHSG) on the surface of a basal-plane pyrolytic graphite electrode (PGE). Since both alkaloids strongly adsorb onto the surface of pyrolytic graphite, measurements were performed using ex situ voltammetric methods, adsorptive transfer (AdT) cyclic voltammetry (CV) and square-wave voltammetry (SWV). The electrochemical results and optimized AdT SWV were subsequently applied for study of the interactions of SG andDHSG with DNA in vitro. Electrochemical study of the interactions was supported and complemented with measurements using gel electrophoresis and fluorescence spectroscopy. The results suggest that SG intercalates into the double-stranded structure of scDNA while increased binding affinity was observed for quaternary cation (SG+). DHSG which, unlike SG+, does not possess a strictly planar molecular structure, did not show intercalative DNA binding in any of the three methods applied.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1671-1680

Kód UT WoS článku

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EID výsledku v databázi Scopus

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