HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10223831" target="_blank" >RIV/61989592:15110/11:10223831 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

Popis výsledku v původním jazyce

We examined whether histone deacetylase (HDAC) inhibitors butyrate and valproate alter expression of NCOR1 and NCOR2 genes coding for N-CoR and SMRT, respectively. Leukemic HL-60 cells were treated for 24 h with 0.5 and 1 mM butyrate, 1-3 mM valproate, 1?M retinoic acid (RA) or cotreated with 1 ?M RA and 0.5 mM butyrate. Histone acetylation was analysed by western blotting. Levels of NCOR1 and NCOR2 mRNA were determined by real-time PCR. Expression of NCF2 gene coding for p67phox was evaluated as myeloid differentiation marker. Butyrate and valproate increased acetylation of histones H3 and H4. They also increased NCF2 mRNA without affecting NCOR1 or NCOR2 mRNA levels. Similarly, RA alone or in combination with butyrate induced NCF2 gene expression without affecting NCOR1 or NCOR2 mRNA levels. We conclude that HDAC inhibitors do not alter expression of NCOR1 or NCOR2 genes and that the onset of myeloid differentiation is not accompanied by induction or repression of these genes in HL-

Název v anglickém jazyce

HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

Popis výsledku anglicky

We examined whether histone deacetylase (HDAC) inhibitors butyrate and valproate alter expression of NCOR1 and NCOR2 genes coding for N-CoR and SMRT, respectively. Leukemic HL-60 cells were treated for 24 h with 0.5 and 1 mM butyrate, 1-3 mM valproate, 1?M retinoic acid (RA) or cotreated with 1 ?M RA and 0.5 mM butyrate. Histone acetylation was analysed by western blotting. Levels of NCOR1 and NCOR2 mRNA were determined by real-time PCR. Expression of NCF2 gene coding for p67phox was evaluated as myeloid differentiation marker. Butyrate and valproate increased acetylation of histones H3 and H4. They also increased NCF2 mRNA without affecting NCOR1 or NCOR2 mRNA levels. Similarly, RA alone or in combination with butyrate induced NCF2 gene expression without affecting NCOR1 or NCOR2 mRNA levels. We conclude that HDAC inhibitors do not alter expression of NCOR1 or NCOR2 genes and that the onset of myeloid differentiation is not accompanied by induction or repression of these genes in HL-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers-Olomouc

ISSN

1213-8118

e-ISSN

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Svazek periodika

155

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

259-262

Kód UT WoS článku

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EID výsledku v databázi Scopus

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