Induction of heme oxygenase-1 by Macleaya cordata extract and its constituent sanguinarine in RAW264.7 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33138582" target="_blank" >RIV/61989592:15110/12:33138582 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Induction of heme oxygenase-1 by Macleaya cordata extract and its constituent sanguinarine in RAW264.7 cells

Popis výsledku v původním jazyce

We examined whether M. cordata extract and its alkaloid constituents, protopine, allocryptopine, sanguinarine and chelerythrine activate Nrf2 signalling pathway which regulates expression of cytoprotective enzymes such as heme oxygenase-1 (HO-1) and thioredoxin 1. In murine RAW264.7 macrophages, M. cordata extract increased mRNA and protein levels of HO-1. Of the alkaloids examined, sanguinarine appeared to be responsible for these effects. Sanguinarine induced nuclear accumulation of Nrf2, increased the expression of Hmox1 gene encoding HO-1 and elevated HO-1 protein levels. Sanguinarine-induced Hmox1 mRNA expression was suppressed by pharmacologic inhibitor of p38 MAPK. Sanguinarine also elevated protein levels of HO-1 and thioredoxin 1 in primary cultures of human hepatocytes. We conclude that sanguinarine may increase the capacity of the enzymatic antioxidant defence system via activation of the p38 MAPK/Nrf2 pathway.

Název v anglickém jazyce

Induction of heme oxygenase-1 by Macleaya cordata extract and its constituent sanguinarine in RAW264.7 cells

Popis výsledku anglicky

We examined whether M. cordata extract and its alkaloid constituents, protopine, allocryptopine, sanguinarine and chelerythrine activate Nrf2 signalling pathway which regulates expression of cytoprotective enzymes such as heme oxygenase-1 (HO-1) and thioredoxin 1. In murine RAW264.7 macrophages, M. cordata extract increased mRNA and protein levels of HO-1. Of the alkaloids examined, sanguinarine appeared to be responsible for these effects. Sanguinarine induced nuclear accumulation of Nrf2, increased the expression of Hmox1 gene encoding HO-1 and elevated HO-1 protein levels. Sanguinarine-induced Hmox1 mRNA expression was suppressed by pharmacologic inhibitor of p38 MAPK. Sanguinarine also elevated protein levels of HO-1 and thioredoxin 1 in primary cultures of human hepatocytes. We conclude that sanguinarine may increase the capacity of the enzymatic antioxidant defence system via activation of the p38 MAPK/Nrf2 pathway.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fitoterapia

ISSN

0367-326X

e-ISSN

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Svazek periodika

83

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

329-335

Kód UT WoS článku

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EID výsledku v databázi Scopus

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