The energetic difference between synthesis of correct and incorrect base pairs accounts for highly accurate DNA replication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33143455" target="_blank" >RIV/61989592:15110/13:33143455 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/ja309866m" target="_blank" >http://dx.doi.org/10.1021/ja309866m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ja309866m" target="_blank" >10.1021/ja309866m</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The energetic difference between synthesis of correct and incorrect base pairs accounts for highly accurate DNA replication

Popis výsledku v původním jazyce

To better understand the energetics of accurate DNA replication, we directly measured deltaG(o) for the incorporation of a nucleotide into elongating dsDNA in solution (deltaG(o)(incorporation)). Direct measurements of the energetic difference between synthesis of correct and incorrect base pairs found it to be much larger than previously believed (average deltadeltaG(o)(incorporation) = 5.2 +- 1.34 kcal mol(-1)). Importantly, these direct measurements indicate that deltadeltaG(o)(incorporation) alone can account for the energy required for highly accurate DNA replication. Evolutionarily, these results indicate that the earliest polymerases did not have to evolve sophisticated mechanisms to replicate nucleic acids; they may only have had to take advantage of the inherently more favorable deltaG(o) for polymerization of correct nucleotides. These results also provide a basis for understanding how polymerases replicate DNA (or RNA) with high fidelity.

Název v anglickém jazyce

The energetic difference between synthesis of correct and incorrect base pairs accounts for highly accurate DNA replication

Popis výsledku anglicky

To better understand the energetics of accurate DNA replication, we directly measured deltaG(o) for the incorporation of a nucleotide into elongating dsDNA in solution (deltaG(o)(incorporation)). Direct measurements of the energetic difference between synthesis of correct and incorrect base pairs found it to be much larger than previously believed (average deltadeltaG(o)(incorporation) = 5.2 +- 1.34 kcal mol(-1)). Importantly, these direct measurements indicate that deltadeltaG(o)(incorporation) alone can account for the energy required for highly accurate DNA replication. Evolutionarily, these results indicate that the earliest polymerases did not have to evolve sophisticated mechanisms to replicate nucleic acids; they may only have had to take advantage of the inherently more favorable deltaG(o) for polymerization of correct nucleotides. These results also provide a basis for understanding how polymerases replicate DNA (or RNA) with high fidelity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of American Chemical Society

ISSN

0002-7863

e-ISSN

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Svazek periodika

135

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

1205-1208

Kód UT WoS článku

000314492500009

EID výsledku v databázi Scopus

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