Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33143791" target="_blank" >RIV/61989592:15110/13:33143791 - isvavai.cz</a>
Výsledek na webu
<a href="http://biomed.papers.upol.cz/pdfs/bio/2013/02/06.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2013/02/06.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2013.036" target="_blank" >10.5507/bp.2013.036</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo
Popis výsledku v původním jazyce
BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is exposed to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm2) or UVB (200 or 800 mJ/cm2). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UVB induced up-regulation of interleukin-1? (IL-1?) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA ca
Název v anglickém jazyce
Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo
Popis výsledku anglicky
BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is exposed to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm2) or UVB (200 or 800 mJ/cm2). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UVB induced up-regulation of interleukin-1? (IL-1?) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA ca
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biomedical Papers-Olomouc
ISSN
1213-8118
e-ISSN
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Svazek periodika
157
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
9
Strana od-do
137-145
Kód UT WoS článku
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EID výsledku v databázi Scopus
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