Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Kód výsledku v IS VaVaI

RIV/61989592:15110/13:33143791 - isvavai.cz

Výsledek na webu

http://biomed.papers.upol.cz/pdfs/bio/2013/02/06.pdf

DOI - Digital Object Identifier

10.5507/bp.2013.036

Jazyk výsledku

angličtina

Název v původním jazyce

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Popis výsledku v původním jazyce

BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is exposed to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm2) or UVB (200 or 800 mJ/cm2). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UVB induced up-regulation of interleukin-1? (IL-1?) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA ca

Název v anglickém jazyce

Differential modulation of inflammatory markers in plasma and skin after single exposures to UVA or UVB radiation in vivo

Popis výsledku anglicky

BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is exposed to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm2) or UVB (200 or 800 mJ/cm2). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UVB induced up-regulation of interleukin-1? (IL-1?) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA ca

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers-Olomouc

ISSN

1213-8118

e-ISSN

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Svazek periodika

157

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

137-145

Kód UT WoS článku

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EID výsledku v databázi Scopus

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