[(68)Ga]NS(3)-RGD and [(68)Ga] Oxo-DO3A-RGD for imaging alfa(v)beta(3) integrin expression: synthesis, evaluation, and comparison

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33145217" target="_blank" >RIV/61989592:15110/13:33145217 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.nucmedbio.2012.09.006" target="_blank" >http://dx.doi.org/10.1016/j.nucmedbio.2012.09.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nucmedbio.2012.09.006" target="_blank" >10.1016/j.nucmedbio.2012.09.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

[(68)Ga]NS(3)-RGD and [(68)Ga] Oxo-DO3A-RGD for imaging alfa(v)beta(3) integrin expression: synthesis, evaluation, and comparison

Popis výsledku v původním jazyce

Introduction: Ga-68-labeled RGD peptides in combination with PET allow non-invasive determination of alpha(v)beta(3) integrin expression which is highly increased during tumor-induced angiogenesis. The aim of this study was to synthesize and evaluate twoRGD peptides containing alternative chelating systems, namely [Ga-68]NS3-RGD and [Ga-68]Oxo-DO3A-RGD and to compare their in vitro and in vivo properties with [Ga-68]DOTA- and [Ga-68]NODAGA-RGD. Methods: Syntheses of both radiotracers followed standardSPPS protocols. For in vitro characterization distribution coefficients, protein binding abilities, serum stablities, and alpha(v)beta(3) integrin binding affinities were determined. For in vitro tests as well as for the biodistribution assay alpha(v)beta(3) positive human melanoma M21 and alpha(v)beta(3) negative M21-L cells were used. Results: Ga-68-labeling of NS3-RGD resulted in good radiochemical purity, whereas HPLC analysis showed two peaks with a ratio of 1:6 for [Ga-68]Oxo-DO3A-

Název v anglickém jazyce

[(68)Ga]NS(3)-RGD and [(68)Ga] Oxo-DO3A-RGD for imaging alfa(v)beta(3) integrin expression: synthesis, evaluation, and comparison

Popis výsledku anglicky

Introduction: Ga-68-labeled RGD peptides in combination with PET allow non-invasive determination of alpha(v)beta(3) integrin expression which is highly increased during tumor-induced angiogenesis. The aim of this study was to synthesize and evaluate twoRGD peptides containing alternative chelating systems, namely [Ga-68]NS3-RGD and [Ga-68]Oxo-DO3A-RGD and to compare their in vitro and in vivo properties with [Ga-68]DOTA- and [Ga-68]NODAGA-RGD. Methods: Syntheses of both radiotracers followed standardSPPS protocols. For in vitro characterization distribution coefficients, protein binding abilities, serum stablities, and alpha(v)beta(3) integrin binding affinities were determined. For in vitro tests as well as for the biodistribution assay alpha(v)beta(3) positive human melanoma M21 and alpha(v)beta(3) negative M21-L cells were used. Results: Ga-68-labeling of NS3-RGD resulted in good radiochemical purity, whereas HPLC analysis showed two peaks with a ratio of 1:6 for [Ga-68]Oxo-DO3A-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nuclear Medicine and Biology

ISSN

0969-8051

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

65-72

Kód UT WoS článku

000312420000009

EID výsledku v databázi Scopus

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