Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33153310" target="_blank" >RIV/61989592:15110/13:33153310 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11046-013-9695-3" target="_blank" >http://dx.doi.org/10.1007/s11046-013-9695-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11046-013-9695-3" target="_blank" >10.1007/s11046-013-9695-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR

Popis výsledku v původním jazyce

Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8 and YTL1-YTR1 are located on 18S subunit of the rRNA gene of P. insidiosum. The specificity, negative and positive predictive values were 100, 100 and 87.5 %, respectively, as compared with direct microscopy and cultivation. The detection limit of the single-tube nested PCR was 21 zoospores corresponding to 2.7 pg of the DNA. The results demonstrate that the new single-tube nested PCR offers a highly sensitive, specific and rapid genetic method for detecting P. insidiosum.

Název v anglickém jazyce

Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR

Popis výsledku anglicky

Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8 and YTL1-YTR1 are located on 18S subunit of the rRNA gene of P. insidiosum. The specificity, negative and positive predictive values were 100, 100 and 87.5 %, respectively, as compared with direct microscopy and cultivation. The detection limit of the single-tube nested PCR was 21 zoospores corresponding to 2.7 pg of the DNA. The results demonstrate that the new single-tube nested PCR offers a highly sensitive, specific and rapid genetic method for detecting P. insidiosum.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MYCOPATHOLOGIA

ISSN

0301-486X

e-ISSN

—

Svazek periodika

176

Číslo periodika v rámci svazku

5-6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

369-376

Kód UT WoS článku

000328194100007

EID výsledku v databázi Scopus

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