Immunomodulatory Effects of Therapeutic Plasma Exchange on Monocytes in Antiphospholipid Syndrome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33156191" target="_blank" >RIV/61989592:15110/16:33156191 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950391/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950391/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/etm.2016.3441" target="_blank" >10.3892/etm.2016.3441</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunomodulatory Effects of Therapeutic Plasma Exchange on Monocytes in Antiphospholipid Syndrome

Popis výsledku v původním jazyce

The antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by thrombosis and recurrent fetal loss, with the persistent presence of antiphospholipid antibodies (aPL). aPL exert their pathogenic effect via overproduction of tissue factor and activation of complement and several cell types, such as endothelia, platelets and importantly also monocytes. As a result, a hypercoagulable state develops leading to APS-associated obstetric complications and fetal loss. Despite far from optimal, treatment of APS usually includes heparin and low dose aspirin. Recently, successful application of plasma exchange (PE) therapy in APS patients with obstetric complications who failed the above standard treatment has been described. In this study we, therefore, investigated mechanism of PE action, namely we explored whether PE affects the functional activity of APS monocytes as assessed by expression of 11 mRNA transcripts (cytokines, signaling molecules/transcription factors). Monocytes were collected before and after the PE course from women with APS who experienced recurrent pregnancy losses as well as healthy volunteers. Compared with control cells, APS monocytes showed deregulated expression of IL-1β, IL-6, IL-23, CCL2, CXCL10 TLR2, and STAT3 at baseline. PE resulted in increased IL-1β, IL-6, IL-23, CCL2, P2X7 and TNFα mRNA transcripts in APS monocytes which returned to normal ranges, similar to those observed in healthy control cells. Furthermore, PE therapy counterbalanced the expression levels of CCL2 and CXCL10 which levels are indicative of Th1/Th2 balance. Thus, our findings provide evidence that primarily altered transcriptional profile of APS monocytes is restored due to immunomodulatory effect of plasmapheresis.

Název v anglickém jazyce

Immunomodulatory Effects of Therapeutic Plasma Exchange on Monocytes in Antiphospholipid Syndrome

Popis výsledku anglicky

The antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by thrombosis and recurrent fetal loss, with the persistent presence of antiphospholipid antibodies (aPL). aPL exert their pathogenic effect via overproduction of tissue factor and activation of complement and several cell types, such as endothelia, platelets and importantly also monocytes. As a result, a hypercoagulable state develops leading to APS-associated obstetric complications and fetal loss. Despite far from optimal, treatment of APS usually includes heparin and low dose aspirin. Recently, successful application of plasma exchange (PE) therapy in APS patients with obstetric complications who failed the above standard treatment has been described. In this study we, therefore, investigated mechanism of PE action, namely we explored whether PE affects the functional activity of APS monocytes as assessed by expression of 11 mRNA transcripts (cytokines, signaling molecules/transcription factors). Monocytes were collected before and after the PE course from women with APS who experienced recurrent pregnancy losses as well as healthy volunteers. Compared with control cells, APS monocytes showed deregulated expression of IL-1β, IL-6, IL-23, CCL2, CXCL10 TLR2, and STAT3 at baseline. PE resulted in increased IL-1β, IL-6, IL-23, CCL2, P2X7 and TNFα mRNA transcripts in APS monocytes which returned to normal ranges, similar to those observed in healthy control cells. Furthermore, PE therapy counterbalanced the expression levels of CCL2 and CXCL10 which levels are indicative of Th1/Th2 balance. Thus, our findings provide evidence that primarily altered transcriptional profile of APS monocytes is restored due to immunomodulatory effect of plasmapheresis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0041" target="_blank" >EE2.3.30.0041: Podpora vytváření excelentních výzkumných týmů a intersektorální mobility na Univerzitě Palackého v Olomouci II.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental and Therapeutic Medicine

ISSN

1792-0981

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

1189-1195

Kód UT WoS článku

000380278900104

EID výsledku v databázi Scopus

2-s2.0-84974739742